A novel role of Mgm1p, a dynamin-related GTPase, in ATP synthase assembly and cristae formation/maintenance

被引：66

作者：

Amutha, B ^{[1
]}

Gordon, DM ^{[1
]}

Gu, YJ ^{[1
]}

Pain, D ^{[1
]}

机构：

[1] UMDNJ, New Jersey Med Sch, Dept Physiol & Pharmacol, Newark, NJ 07101 USA

来源：

BIOCHEMICAL JOURNAL | 2004年 / 381卷

关键词：

ATP synthase; cytochrome c; Mgm1p; mitochondria; rhomboid-like protease; Tim11p;

D O I：

10.1042/BJ20040566

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

In Saccharomyces cerevisiae, two mitochondrial inner-membrane proteins play critical roles in organellar morphology. One is a dynamin-related GTPase, Mgm1p, which participates in mitochondrial fusion. Another is Tim11p, which is required for oligomeric assembly of F1Fo-ATP synthase, which generates ATP through oxidative phosphorylation. Our data bring these findings together and define a novel role for Mgm1p in the formation and maintenance of mitochondrial cristae. We show that Mgm1p serves as an upstream regulator of Tim11p protein stability, ATP synthase assembly, cristae morphology and cytochrome c storage within cristae.

引用

页码：19 / 23

页数：5

共 30 条

[1]

OPA1, encoding a dynamin-related GTPase, is mutated in autosomal dominant optic atrophy linked to chromosome 3q28 [J].

Alexander, C ;

Votruba, M ;

Pesch, UEA ;

Thiselton, DL ;

Mayer, S ;

Moore, A ;

Rodriguez, M ;

Kellner, U ;

Leo-Kottler, B ;

Auburger, G ;

Bhattacharya, SS ;

Wissinger, B .

NATURE GENETICS, 2000, 26 (02) :211-215

[2]

Nucleoside diphosphate kinase of Saccharomyces cerevisiae, Ynk1p:: localization to the mitochondrial intermembrane space [J].

Amutha, B ;

Pain, D .

BIOCHEMICAL JOURNAL, 2003, 370 (03) :805-815

[3]

Yeast mitochondrial F1F0-ATP synthase exists as a dimer:: identification of three dimer-specific subunits [J].

Arnold, I ;

Pfeiffer, K ;

Neupert, W ;

Stuart, RA ;

Schägger, H .

EMBO JOURNAL, 1998, 17 (24) :7170-7178

[4]

Yeast mitochondrial F1F0-ATPase: the novel subunit e is identical Tim11 [J].

Arnold, I ;

Bauer, MF ;

Brunner, M ;

Neupert, W ;

Stuart, RA .

FEBS LETTERS, 1997, 411 (2-3) :195-200

[5]

Bleazard, W ;

McCaffery, JM ;

King, EJ ;

Bale, S ;

Mozdy, A ;

Tieu, Q ;

Nunnari, J ;

Shaw, JM .

NATURE CELL BIOLOGY, 1999, 1 (05) :298-304

[6]

Regulated intramembrane proteolysis: A control mechanism conserved from bacteria to humans [J].

Brown, MS ;

Ye, J ;

Rawson, RB ;

Goldstein, JL .

CELL, 2000, 100 (04) :391-398

[7]

Su e of the yeast F1Fo-ATP synthase forms homodimers [J].

Brunner, S ;

Everard-Gigot, V ;

Stuart, RA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (50) :48484-48489

[8]

Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy [J].

Delettre, C ;

Lenaers, G ;

Griffoin, JM ;

Gigarel, N ;

Lorenzo, C ;

Belenguer, P ;

Pelloquin, L ;

Grosgeorge, J ;

Turc-Carel, C ;

Perret, E ;

Astarie-Dequeker, C ;

Lasquellec, L ;

Arnaud, B ;

Ducommun, B ;

Kaplan, J ;

Hamel, CP .

NATURE GENETICS, 2000, 26 (02) :207-210

[9]

FOX TD, 1991, METHOD ENZYMOL, V194, P149

[10]

Loss of the intermembrane space protein Mgm1/OPA1 induces swelling and localized constrictions along the lengths of mitochondria [J].

Griparic, L ;

van der Wel, NN ;

Orozco, IJ ;

Peters, PJ ;

van der Bliek, AM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (18) :18792-18798

← 1 2 3 →