Biochemical and structural characterization of 5′-methylthioadenosine nucleosidases from Arabidopsis thaliana

被引：5

作者：

Park, Eun Young ^{[1
]}

Choi, Woo Suk ^{[1
]}

Oh, Seung-Ick ^{[1
]}

Kim, Kyung-Nam ^{[2
]}

Shin, Jeong Sheop ^{[1
]}

Song, Hyun Kyu ^{[1
]}

机构：

[1] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea

[2] Sejong Univ, Dept Mol Biol, Seoul 143747, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 381卷 / 04期

关键词：

Adenine; Crystal structure; Flexible loop; MTA; Nucleosidase; Plant; SAH; Spectroscopic assay; COLI 5'-METHYLTHIOADENOSINE/S-ADENOSYLHOMOCYSTEINE NUCLEOSIDASE; TRANSITION-STATE ANALOGS; ESCHERICHIA-COLI; MTA/ADOHCY NUCLEOSIDASE; S-ADENOSYLMETHIONINE; ETHYLENE SYNTHESIS; FRUIT-DEVELOPMENT; BIOSYNTHESIS; POLYAMINE; PLANTS;

D O I：

10.1016/j.bbrc.2009.02.106

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5'-Methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) are important metabolites in all living organisms. Two similar nucleosidases for hydrolyzing MTA in Arabidopsis thaliana (AtMTAN1 and AtMTAN2) exist, but only AtMTAN2 shows markedly broad substrate specificity for hydrolysis of SAH. To examine the biochemical characteristics of AtMTAN2, it was over-expressed in Escherichia coli and purified to homogeneity. Spectroscopic assays confirm AtMTAN2 catalyzes MTA as well as SAH hydrolysis, compared to AtMTAN1 which only hydrolyzes MTA. In addition, crystal structure of the AtMTAN2 enzyme in complex with, adenine was determined at 2.9 angstrom resolution. Finally, a structural comparison of AtMTAN2 performed with previously determined structures of AtMTAN1 and an E. coli homolog provides clues for the substrate specificity of MTA nucleosidases in A. thaliana. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：619 / 624

页数：6

共 29 条

[1] ALSCRIPT - A TOOL TO FORMAT MULTIPLE SEQUENCE ALIGNMENTS [J].

BARTON, GJ .

PROTEIN ENGINEERING, 1993, 6 (01) :37-40

[2] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[3] S-adenosylmethionine and methylation [J].

Chiang, PK ;

Gordon, RK ;

Tal, J ;

Zeng, GC ;

Doctor, BP ;

Pardhasaradhi, K ;

McCann, PP .

FASEB JOURNAL, 1996, 10 (04) :471-480

[4] Coot:: model-building tools for molecular graphics [J].

Emsley, P ;

Cowtan, K .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 :2126-2132

[5] REDUCED ETHYLENE SYNTHESIS BY TRANSGENIC TOMATOES EXPRESSING S-ADENOSYLMETHIONINE HYDROLASE [J].

GOOD, X ;

KELLOGG, JA ;

WAGONER, W ;

LANGHOFF, D ;

MATSUMURA, W ;

BESTWICK, RK .

PLANT MOLECULAR BIOLOGY, 1994, 26 (03) :781-790

[6] 5'-METHYLTHIOADENOSINE NUCLEOSIDASE - PURIFICATION AND CHARACTERIZATION OF THE ENZYME FROM LUPINUS-LUTEUS SEEDS [J].

GURANOWSKI, AB ;

CHIANG, PK ;

CANTONI, GL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 114 (02) :293-299

[7] IMPROVED METHODS FOR BUILDING PROTEIN MODELS IN ELECTRON-DENSITY MAPS AND THE LOCATION OF ERRORS IN THESE MODELS [J].

JONES, TA ;

ZOU, JY ;

COWAN, SW ;

KJELDGAARD, M .

ACTA CRYSTALLOGRAPHICA SECTION A, 1991, 47 :110-119

[8] 5'-METHYLTHIOADENOSINE NUCLEOSIDASE AND 5-METHYLTHIORIBOSE KINASE-ACTIVITIES AND ETHYLENE PRODUCTION DURING TOMATO FRUIT-DEVELOPMENT AND RIPENING [J].

KUSHAD, MM ;

RICHARDSON, DG ;

FERRO, AJ .

PLANT PHYSIOLOGY, 1985, 79 (02) :525-529

[9] INTERRELATIONSHIP OF POLYAMINE AND ETHYLENE BIOSYNTHESIS DURING AVOCADO FRUIT-DEVELOPMENT AND RIPENING [J].

KUSHAD, MM ;

YELENOSKY, G ;

KNIGHT, R .

PLANT PHYSIOLOGY, 1988, 87 (02) :463-467

[10] 5-METHYLTHIORIBOSE KINASE-ACTIVITY IN PLANTS [J].

KUSHAD, MM ;

RICHARDSON, DG ;

FERRO, AJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 108 (01) :167-173

← 1 2 3 →