Molecular and functional characterization of an amphibian urea transporter

被引：37

作者：

Couriaud, C

Leroy, C

Simon, M

Silberstein, C

Bailly, P

Ripoche, P

Rousselet, G

机构：

[1] CEA Saclay, Serv Biol Cellulaire, F-91191 Gif Sur Yvette, France

[2] Univ Buenos Aires, Fac Med, Dept Fisiol, RA-1425 Buenos Aires, DF, Argentina

[3] INSERM U76, GIP, Ins Natl Transfus Sanguine, Paris, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1999年 / 1421卷 / 02期

关键词：

urea transporter; phloretin; thiourea; Rana esculenta;

D O I：

10.1016/S0005-2736(99)00147-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the characterization of a frog (Rana esculenta) urea transporter (fUT). The cloned cDNA is 1.4 kb long and contains a putative open reading frame of 1203 bp. In frog urinary bladder, the gene is expressed as two mRNAs of 4.3 and 1.6 kb. The fUT protein is 63.1 and 56.3% identical to rat UT-A2 and UT-B1, respectively. The internal duplication of UT-A2 and UT-B, as well as the double LP box urea transporter signature sequence were found in this amphibian urea transporter. When expressed in Xenopus oocytes, fUT induced a 10-fold increase in urea permeability, which was blocked by both phloretin and mercurial reagents. The fUT protein did not transport thiourea, but the fUT-mediated urea transport was strongly inhibited by this compound. Thus, this amphibian urea transporter displays transport characteristics in between those of UT-A2 and UT-B. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：347 / 352

页数：6

共 30 条

[21] Tandem sequence repeats in urea transporters: Identification, of an urea transporter signature sequence [J].