Determination of mobile phase effect on single-component adsorption isotherm by use of numerical estimation

被引：35

作者：

Antos, D ^{[1
]}

Piatkowski, W ^{[1
]}

Kaczmarski, K ^{[1
]}

机构：

[1] Rzeszow Univ Technol, Dept Chem Engn, Fac Chem, Rzeszow, Poland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 874卷 / 01期

关键词：

adsorption isotherms; mobile phase composition; band profiles; overload gradient elution; preparative chromatography;

D O I：

10.1016/S0021-9673(00)00082-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerical estimation was used to determine adsorption isotherm parameters of a single-component in a normal-phase system. The distribution isotherm of methyl deoxycholate was described between the mobile phase containing hexane, ethyl acetate, methanol with varied concentration and a silica gel adsorbent. The effect of the mobile phase composition on the isotherm parameters and the band profiles was investigated. The results obtained were used to simulate the overload gradient elution. The validity of the method proposed was verified by comparison of the computer simulations with the experimental band profiles. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 12

页数：12

共 23 条

[11]

GUIOCHON G, 1994, FUNDAMENALS PREPARAT

[12] Determination of binary competitive equilibrium isotherms from the individual chromatographic band profiles [J].

James, F ;

Sepúlveda, M ;

Charton, F ;

Quinones, I ;

Guiochon, G .

CHEMICAL ENGINEERING SCIENCE, 1999, 54 (11) :1677-1696

[13] Characterisation of retention in micellar high-performance liquid chromatography and in micellar electrokinetic chromatography using lipophilicity and polarity indices [J].

Jandera, P ;

Fischer, J ;

Effenberger, H .

JOURNAL OF CHROMATOGRAPHY A, 1998, 807 (01) :57-70

[14] Effects of the gradient profile on the production rate in reversed-phase gradient elution overloaded chromatography [J].

Jandera, P ;

Komers, D ;

Guiochon, G .

JOURNAL OF CHROMATOGRAPHY A, 1997, 760 (01) :25-39

[15] Optimization of the recovery yield and of the production rate in overloaded gradient-elution reversed-phase chromatography [J].

Jandera, P ;

Komers, D ;

Guiochon, G .

JOURNAL OF CHROMATOGRAPHY A, 1998, 796 (01) :115-127

[16] GRADIENT ELUTION IN LIQUID-CHROMATOGRAPHY .8. SELECTION OF OPTIMAL COMPOSITION OF MOBILE PHASE IN LIQUID-CHROMATOGRAPHY UNDER ISOCRATIC CONDITIONS [J].

JANDERA, P ;

JANDEROVA, M ;

CHURACEK, J .

JOURNAL OF CHROMATOGRAPHY, 1978, 148 (01) :79-97

[17] Effects of the composition of the mobile phase on the production rate in reversed-phase overloaded chromatography [J].

Jandera, P ;

Komers, D ;

Guiochon, G .

JOURNAL OF CHROMATOGRAPHY A, 1997, 787 (1-2) :13-25

[18] Optimisation of gradient elution in normal-phase high-performance liquid chromatography [J].

Jandera, P .

JOURNAL OF CHROMATOGRAPHY A, 1998, 797 (1-2) :11-22

[19] Use of orthogonal collocation on finite elements with moving boundaries in the simulation of non-linear multicomponent chromatography. Influence of fluid velocity variation on retention time in LC and HPLC [J].

Kaczmarski, K .

COMPUTERS & CHEMICAL ENGINEERING, 1996, 20 (01) :49-64

[20] Modeling fixed-bed adsorption columns through orthogonal collocations on moving finite elements [J].

Kaczmarski, K ;

Mazzotti, M ;

Storti, G ;

Morbidelli, M .

COMPUTERS & CHEMICAL ENGINEERING, 1997, 21 (06) :641-660

← 1 2 3 →