Spontaneous membrane fusion induced by chemical formation of ceramides in a lipid bilayer

被引:22
作者
Kunishima, Munetaka [1 ]
Tokaji, Masafumi
Matsuoka, Keisuke
Nishida, Jin
Kanamori, Masanori
Hioki, Kazuhito
Tani, Shohei
机构
[1] Kobe Gakuin Univ, Fac Pharmaceut Sci, Nishi Ku, Kobe, Hyogo 6512180, Japan
[2] JST, PRESTO, Nishi Ku, Kobe, Hyogo 6512180, Japan
关键词
D O I
10.1021/ja0652969
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Mere chemical generation of ceramide and related double-chain lipids in the membrane of small unilamellar vesicles (SUVs) induces fusion of the vesicles. The lipids can be successfully prepared by dehydrocondensation between single-chain lipids (fatty acids and sphingosine or its analogues) in a lipid bilayer of the SUV by using a combination of 2-chloro-4,6-dimethoxy-1,3,5-triazine and amphiphilic tertiary amine catalysts, a process that can be compared to a successive enzyme model system for a fatty acyl-CoA synthetase followed by acyltransferase. The SUV spontaneously undergoes membrane fusion upon this internal chemical stimulation by the artificial enzyme system. Copyright © 2006 American Chemical Society.
引用
收藏
页码:14452 / 14453
页数:2
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