A microfluidic device to study cancer metastasis under chronic and intermittent hypoxia

被引：49

作者：

Acosta, Miguel A. ^{[1
]}

Jiang, Xiao ^{[1
]}

Huang, Pin-Kang ^{[2
]}

Cutler, Kyle B. ^{[3
]}

Grant, Christine S. ^{[1
]}

Walker, Glenn M. ^{[1
]}

Gamcsik, Michael P. ^{[2
]}

机构：

[1] N Carolina State Univ, UNC NCSU Joint Dept Biomed Engn, Raleigh, NC 27695 USA

[2] Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei 106, Taiwan

[3] Univ Calif Irvine, Beckman Laser Inst, Dept Biomed Engn, Irvine, CA 92617 USA

来源：

BIOMICROFLUIDICS | 2014年 / 8卷 / 05期

关键词：

EPITHELIAL-MESENCHYMAL TRANSITION; CELL-CULTURE; TISSUE-CULTURE; OXYGEN LEVELS; TUMOR; MIGRATION; PLATFORM; MODEL; GRADIENTS; INVASION;

D O I：

10.1063/1.4898788

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Metastatic cancer cells must traverse a microenvironment ranging from extremely hypoxic, within the tumor, to highly oxygenated, within the host's vasculature. Tumor hypoxia can be further characterized by regions of both chronic and intermittent hypoxia. We present the design and characterization of a microfluidic device that can simultaneously mimic the oxygenation conditions observed within the tumor and model the cell migration and intravasation processes. This device can generate spatial oxygen gradients of chronic hypoxia and produce dynamically changing hypoxic microenvironments in long-term culture of cancer cells. (C) 2014 AIP Publishing LLC.

引用

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