Unexpected observation of ion suppression in a liquid chromatography/atmospheric pressure chemical ionization mass spectrometric bioanalytical method

被引：58

作者：

Sangster, T ^{[1
]}

Spence, M ^{[1
]}

Sinclair, P ^{[1
]}

Payne, R ^{[1
]}

Smith, C ^{[1
]}

机构：

[1] AstraZeneca, DMPK Alderley, Macclesfield SK10 4TG, Cheshire, England

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 12期

关键词：

D O I：

10.1002/rcm.1477

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ion suppression is a well-known phenomenon in electrospray ionization (ESI) mass spectrometry. These suppression effects have been shown to adversely affect the accuracy and precision of quantitative bioanalytical methods using ion spray. Such suppression effects have not been as well defined in atmospheric pressure chemical ionization (APCI) and there is some debate whether these effects actually occur in the ionization process using APCI. Here an example is described where clear ion suppression was observed during studies on a model compound and three metabolites using APCI liquid chromatography/tandem mass spectrometry (LC/MS/MS). Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：1361 / 1364

页数：4

共 13 条

[1] Amad MH, 2000, J MASS SPECTROM, V35, P784, DOI 10.1002/1096-9888(200007)35:7<784::AID-JMS17>3.0.CO
[2] 2-Q
[3] Bonfiglio R, 1999, RAPID COMMUN MASS SP, V13, P1175, DOI 10.1002/(SICI)1097-0231(19990630)13:12<1175::AID-RCM639>3.3.CO
[4] 2-S
[5] Practical implications of some recent studies in electrospray ionization fundamentals
Cech, NB
Enke, CG
[J]. MASS SPECTROMETRY REVIEWS, 2001, 20 (06) : 362 - 387
[6] Clarke S.D., 1996, PHARM SCI, V2, P203
[7] Kebarle P, 2000, J MASS SPECTROM, V35, P804, DOI 10.1002/1096-9888(200007)35:7<804::AID-JMS22>3.0.CO
[8] 2-Q
[9] Mechanistic investigation of ionization suppression in electrospray ionization
King, R
Bonfiglio, R
Fernandez-Metzler, C
Miller-Stein, C
Olah, T
[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2000, 11 (11) : 942 - 950
[10] Law B, 2003, HANDB ANALYT SEPARAT, V4, P271

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