The BldD protein from Streptomyces coelicolor is a DNA-binding protein

被引：39

作者：

Elliot, MA ^{[1
]}

Leskiw, BK ^{[1
]}

机构：

[1] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 21期

关键词：

D O I：

10.1128/JB.181.21.6832-6835.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Gel mobility shift assays with His tagged BldD isolated from Escherichia coli have illustrated that BldD is capable of specifically recognizing its own promoter region. DNase I and hydroxyl radical footprinting assays have served to delimit the BldD binding site, revealing that BldD recognizes and binds to a site just upstream from, and overlapping with, the -10 region of the promoter. How BldD binds to its promoter and the effect this binding has on the expression of BldD are discussed.

引用

页码：6832 / 6835

页数：4