ERIS, an endoplasmic reticulum IFN stimulator, activates innate immune signaling through dimerization

被引：682

作者：

Sun, Wenxiang ^{[1
]}

Li, Yang ^{[1
]}

Chen, Lu ^{[1
]}

Chen, Huihui ^{[1
]}

You, Fuping ^{[1
]}

Zhou, Xiang ^{[1
]}

Zhou, Yi ^{[1
]}

Zhai, Zhonghe ^{[1
]}

Chen, Danying ^{[1
]}

Jiang, Zhengfan ^{[1
]}

机构：

[1] Peking Univ, Sch Life Sci, Educ Minist, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 21期

基金：

中国国家自然科学基金;

关键词：

innate immunity; type I IFN; functional cDNA library screening; cytosolic RNA and dsDNA; ER retention signal; NF-KAPPA-B; DOUBLE-STRANDED-RNA; RIG-I; ADAPTER PROTEIN; ANTIVIRAL RESPONSE; MEMBRANE-PROTEINS; VIRUS; RECOGNITION; RECEPTORS; DNA;

D O I：

10.1073/pnas.0900850106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report here the identification and characterization of a protein, ERIS, an endoplasmic reticulum (ER) IFN stimulator, which is a strong type I IFN stimulator and plays a pivotal role in response to both non-self-cytosolic RNA and dsDNA. ERIS (also known as STING or MITA) resided exclusively on ER membrane. The ER retention/retrieval sequence RIR was found to be critical to retain the protein on ER membrane and to maintain its integrity. ERIS was dimerized on innate immune challenges. Coumermycin-induced ERIS dimerization led to strong and fast IFN induction, suggesting that dimerization of ERIS was critical for self-activation and subsequent downstream signaling.

引用

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页码：8653 / 8658

页数：6

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