Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France

被引:248
作者
Pradel, N
Livrelli, V
De Champs, C
Palcoux, JB
Reynaud, A
Scheutz, F
Sirot, J
Joly, B
Forestier, C
机构
[1] Univ Clermont Ferrand 1, Fac Pharm, Grp Rech Pathogene Bacterienne Intestinale, F-63001 Clermont Ferrand, France
[2] CHU, Fac Med, Bacteriol Lab, Serv Pediat, Clermont Ferrand, France
[3] Lab Departemental Anal Vet & Biol, Clermont Ferrand, France
[4] Statens Serum Inst, Int Escherichia Ctr WHO, DK-2300 Copenhagen, Denmark
关键词
D O I
10.1128/JCM.38.3.1023-1031.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (10%) of 603 cheese samples, and 19 (3%) of 658 stool specimens from hospitalized children with and without diarrhea were positive for the sh gene(s). A STEC strain was isolated from 34% (162 of 471) of bovine feces, 4% (16 of 411) of beef samples, 1% (5 of 603) of cheese samples, and 1.5% (10 of 658) of stool specimens. Of the 220 STEC strains isolated, 34 (15%) harbored the stx(1) gene, 116 (53%) harbored the stx(2) gene, and 70 (32%) carried both the stx(1) and stx(2) genes. However, 32 (14.5%) were not cytotoxic for Vero cells. The eae gene, found in 12 (5%) of the 220 strains, was significantly associated with the stx(1) gene and with isolates from children. Sequences homologous to ehxA were found in 102 (46%) of the 220 strains. Thirteen serotypes, OX3:H2, O113:H21, O113:H4, OX3:H21, O6:H10, OX178:H19, O171:H2, O46:H38, O172:H21, O22:H16, O91:H10, O91:H21, and O22:H8, accounted for 102 (55%) of 186 typeable isolates, and only one strain (0.5% of the 186 STEC isolates from cattle), belonged to the O157:H7 serotype. We showed that the majority of the STEC isolates from cattle, beef, and cheese were not likely to be pathogenic for humans and that the STEC strains isolated from children in this study were probably not responsible for diarrheal disease. Finally, the strains associated with hemolytic-uremic syndrome in the same geographical area were shown to belong to particular subsets of the STEC population found in the bovine reservoir.
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页码:1023 / 1031
页数:9
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