Effect of mobile phase additives in packed-column subcritical and supercritical fluid chromatography

被引:115
作者
Blackwell, JA [1 ]
Stringham, RW [1 ]
Weckwerth, JD [1 ]
机构
[1] UNIV MINNESOTA,DEPT CHEM,MINNEAPOLIS,MN 55455
关键词
D O I
10.1021/ac9608883
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The effect of mobile phase additives is investigated for a variety of compounds under subcritical and supercritical conditions using packed columns. Retention of hydrogen bond donor/acceptor analytes was found to be more dependent on the presence of mobile phase additives than weak hydrogen bond acceptor analytes. The temperature and pressure of the mobile phase are major factors in the extent of this dependence. Consequently, selectivity between homologous compounds is dependent on both the additive used and the state of the mobile phase. Efficiency is nearly always improved by the presence of mobile phase additives, more so under supercritical conditions than under subcritical conditions. These observations suggest that surface molar excesses of mobile phase additives play a large part in the resulting character of the supercritical chromatographic system.
引用
收藏
页码:409 / 415
页数:7
相关论文
共 26 条
[2]   A COMPARISON OF LC AND SFC FOR CELLULOSE-DERIVED AND AMYLOSE-DERIVED CHIRAL STATIONARY PHASES [J].
BARGMANNLEYDER, N ;
TAMBUTE, A ;
CAUDE, M .
CHIRALITY, 1995, 7 (05) :311-325
[3]   ROLE OF ADDITIVES IN PACKED-COLUMN SUPERCRITICAL FLUID CHROMATOGRAPHY - SUPPRESSION OF SOLUTE IONIZATION [J].
BERGER, TA ;
DEYE, JF .
JOURNAL OF CHROMATOGRAPHY, 1991, 547 (1-2) :377-392
[4]   SEPARATION OF HYDROXYBENZOIC ACIDS BY PACKED-COLUMN SUPERCRITICAL FLUID CHROMATOGRAPHY USING MODIFIED FLUIDS WITH VERY POLAR ADDITIVES [J].
BERGER, TA ;
DEYE, JF .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1991, 29 (01) :26-30
[5]  
BERGER TA, 1991, J CHROMATOGR SCI, V29, P319
[6]   USE OF A NEW PIRKLE-TYPE CHIRAL STATIONARY-PHASE IN ANALYTICAL AND PREPARATIVE SUBCRITICAL FLUID CHROMATOGRAPHY OF PHARMACEUTICAL COMPOUNDS [J].
BLUM, AM ;
LYNAM, KG ;
NICOLAS, EC .
CHIRALITY, 1994, 6 (04) :302-313
[7]   Effect of various modifiers on selectivity in packed column subcritical and supercritical fluid chromatography [J].
Cantrell, GO ;
Stringham, RW ;
Blackwell, JA ;
Weckwerth, JD ;
Carr, PW .
ANALYTICAL CHEMISTRY, 1996, 68 (20) :3645-3650
[8]   SOLVATOCHROMISM, LINEAR SOLVATION ENERGY RELATIONSHIPS, AND CHROMATOGRAPHY [J].
CARR, PW .
MICROCHEMICAL JOURNAL, 1993, 48 (01) :4-28
[9]   VAPOR-LIQUID EQUILIBRIA AT HIGH PRESSURES - CALCULATION OF CRITICAL TEMPERATURES VOLUMES AND PRESSURES OF NONPOLAR MIXTURES [J].
CHUEH, PL ;
PRAUSNIT.JM .
AICHE JOURNAL, 1967, 13 (06) :1107-&
[10]  
Dean J.A., 1985, LANGES HDB CHEM, V13