The use of random amplified polymorphic DNA (RAPD) markers to identify strawberry varieties: a forensic application

被引:70
作者
Congiu, L
Chicca, M
Cella, R
Rossi, R
Bernacchia, G
机构
[1] Univ Ferrara, Dept Biol, I-44100 Ferrara, Italy
[2] Univ Pavia, Dept Genet & Microbiol, I-27100 Pavia, Italy
关键词
cultivar identification; DNA extraction; forensic; patented variety; RAPD; strawberry;
D O I
10.1046/j.1365-294x.2000.00811.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The random amplified polymorphic DNA (RAPD) technique was applied to settle a lawsuit involving unauthorized commercialization of a patented strawberry variety of high economical relevance ('Marmolada'(R)). Because of economical involvements, the molecular approach was added to the more traditional morphological examination in a double-blind test. All plants belonging to the patented variety were unambiguously identified (13 plants among a total of 31 plants examined). The results were accepted as evidence in the court. This study confirms that the RAPD technique is especially suitable for identification of asexually reproduced plant varieties for forensic or agricultural purposes.
引用
收藏
页码:229 / 232
页数:4
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