Species determination - Can we detect and quantify meat adulteration?

被引:235
作者
Ballin, Nicolai Z. [1 ,2 ]
Vogensen, Finn K. [1 ]
Karlsson, Anders H. [1 ]
机构
[1] Univ Copenhagen, Fac Life Sci, Dept Food Sci, DK-1958 Frederiksberg C, Denmark
[2] Danish Vet & Food Adm, Reg Vet & Food Control Author, Dept Food Chem, DK-4100 Ringsted, Denmark
关键词
Adulteration; Authentication; Fraud; Meat; Meat products; Real time PCR; Speciation; Species determination; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; INTERSPERSED REPETITIVE ELEMENTS; LINKED-IMMUNOSORBENT-ASSAY; PROCESSED ANIMAL PROTEINS; BOVINE-DERIVED MATERIALS; MITOCHONDRIAL-DNA; BONE MEAL; QUANTITATIVE PCR; LIQUID-CHROMATOGRAPHY;
D O I
10.1016/j.meatsci.2009.06.003
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Proper labelling of meat products is important to help fair-trade, and to enable consumers to make informed choices. However, it has been shown that labelling of species, expressed as weight/weight (w/w), on meat product labels was incorrect in more than 20% of cases. Enforcement of labelling regulations requires reliable analytical methods. Analytical methods are often based on protein or DNA measurements, which are not directly comparable to labelled meat expressed as w/w. This review discusses a wide range of analytical methods with focus on their ability to quantify and their limits of detection (LOD). in particular, problems associated with a correlation from quantitative DNA based results to meat content (w/w) are discussed. The hope is to make researchers aware of the problems of expressing DNA results as meat content (w/w) in order to find better alternatives. One alternative is to express DNA results as genome/genome equivalents. (C) 2009 Elsevier Ltd. All rights reserved.
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页码:165 / 174
页数:10
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