Fluorescence measurement of glucose by pyrene-modified oxidase

被引：15

作者：

Tatsu, Y ^{[1
]}

Yamamura, S ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Ikeda, Osaka 5638577, Japan

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2002年 / 17卷 / 3-5期

关键词：

glucose; glucose oxidase; fluorescence; pyrene;

D O I：

10.1016/S1381-1177(02)00029-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucose oxidase was chemically modified with pyrene. The fluorescence intensity of the modified oxidase increased by glucose. The increase was attributed to the enzymatic consumption of dissolved oxygen that quenched the fluorescence of pyrene. Glucose concentration was measured quantitatively from 1 to 17 mM by the fluorescence measurement. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：203 / 206

页数：4

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