Genotoxic effects of stannous chloride (SnCl2) in K562 cell line

被引：29

作者：

Dantas, FJS

de Mattos, JCP

Moraes, MO

Viana, ME

Lage, CAS

Cabral-Neto, JB

Leitao, AC

Bernardo, M

Bezerra, RJAC

Carvalho, JJ

Caldeira-de-Araújo, A

机构：

[1] UEFJ, IBRAG, Dept Biofis & Biometria, BR-20551030 Rio De Janeiro, RJ, Brazil

[2] Fiocruz MS, IOC, Lab Hanseniase, BR-21045900 Rio De Janeiro, RJ, Brazil

[3] Univ Fed Rio de Janeiro, IBCCF, Lab Radiobiol Mol, BR-21949900 Rio de Janeiro, RJ, Brazil

[4] Univ Estado Rio De Janeiro, IBRAG, Dept Histol & Embriol, BR-20551030 Rio De Janeiro, RJ, Brazil

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2002年 / 40卷 / 10期

关键词：

SnCl2; K562; DNA damage; trypan blue; comet assay;

D O I：

10.1016/S0278-6915(02)00087-X

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The toxic effects of SnCl2 in K562 cells were analyzed in this study. This cell line is resistant to reactive oxygen species (ROS) making it suitable to evaluate the impact of SnCl2 in culture either through ROS or by direct toxicity using Trypan blue dye exclusion, comet and flow cytometry assays. An important loss of viability induced by SnCl2 in a dose-response manner was observed in cells treated in Tris-buffered saline (TBS). This necrotic cell death was further confirmed by flow cytometry. On the other hand, there was no loss of viability when cells were treated in rich medium (RPMI). DNA damage was visualized in SnCl2-treated K562 cells in both tested conditions. The data indicate that SnCl2 induces DNA damage and reduces K562 viability. Both actions seem to be correlated with ROS formation and direct linkage to DNA. (C) 2002 Published by Elsevier Science Ltd.

引用

页码：1493 / 1498

页数：6

共 26 条

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