Detecting transcriptionally active regions using genomic tiling arrays

被引：19

作者：

Halasz, Gabor

van Batenburg, Marinus F.

Perusse, Joelle

Hua, Sujun

Lu, Xiang-Jun

White, Kevin P.

Bussemaker, Harmen J.

机构：

[1] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

[2] Columbia Univ, Integrated Cellular Mol & Biophys Studies, New York, NY 10032 USA

[3] Univ Amsterdam, Acad Med Ctr, Bioinformat Lab, NL-1105 AZ Amsterdam, Netherlands

[4] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06520 USA

[5] Yale Univ, Dept Ecol & Evolut Biol, New Haven, CT 06250 USA

[6] Columbia Univ, Ctr Computat Biol & Bioinformat, New York, NY USA

来源：

GENOME BIOLOGY | 2006年 / 7卷 / 07期

关键词：

D O I：

10.1186/gb-2006-7-7-r59

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a method for interpreting genomic tiling array data, implemented as the program TranscriptionDetector. Probed loci expressed above background are identified by combining replicates in a way that makes minimal assumptions about the data. We performed medium-resolution Anopheles gambiae tiling array experiments and found extensive transcription of both coding and non-coding regions. Our method also showed improved detection of transcriptional units when applied to high-density tiling array data for ten human chromosomes.

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页数：10

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