An enzyme-modified chemiluminescence detector for hydrogen peroxide and oxidase substrates

Enzyme-modified silica and graphite pastes have been used to construct chemiluminescence detectors for hydrogen peroxide, glucose and L-lactate. Fungal peroxidase (FRP) is immobilized in the pastes to detect hydrogen peroxide rapidly with high sensitivity in the range between 1 mu M and 1 mM. The corresponding graphite paste combines high sensitivity with an acceptable signal stability and is coated with poly-o-phenylenediamine by electropolymerization. Glucose oxidase (GOD) and lactate oxidase (LOD) are immobilized in a poly(carbamoylsulphonate) hydrogel, which is adsorbed on the FRP-modified graphite paste. The bienzyme optrodes thus obtained show rapid response for glucose and L-lactate in the range between 50 mu M and 8 mM.