Immunoactive polysaccharide-rich fractions from Panax notoginseng

被引：33

作者：

Zhu, Ying

Pettolino, Filomena

Mau, Shaio-lim

Shen, Yu-Chang

Chen, Chie-Fu

Kuo, Yuh-Chi

Bacic, Antony ^{[1
]}

机构：

[1] Univ Melbourne, Sch Bot, Cooperat Res Ctr Bioprod, Melbourne, Vic 3010, Australia

[2] Natl Res Inst Chinese Med, Taipei, Taiwan

[3] Fu Jen Catholic Univ, Inst Life Sci, Taipei, Taiwan

来源：

PLANTA MEDICA | 2006年 / 72卷 / 13期

关键词：

Panax notoginseng; Araliaceae; polysaccharides; complement-fixing activity; ROS production; mitogenic effect; immunomodulation;

D O I：

10.1055/s-2006-947222

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Panax notoginseng is a commonly used medicinal plant in southwestern China. In a previous study, a sequential solubilisation of P. notoginseng high-molecular-weight (HMW) polymers using phenol-acetic acid-water, hot water, weak and strong alkali was performed to determine the structure of the component polysaccharides and proteins. The effects of these extracted HMW fractions on the human complement system, polymorphonuclear neutrophils (PMN) and peripheral blood mononuclear cells (PBMC) are reported here. Fr-1MKOH, which was extracted with 1 M KOH, showed the strongest complement-fixing activity and priming of reactive oxygen species (ROS) production by PMNs, as well as a mitogenic effect. Fr-1MKOH was further fractionated by anion-exchange chromatography followed by gel-permeation chromatography. 1MD3-G2, the fraction most strongly bound to the DEAE anion-exchange column with a molecular weight of 1140 kDa, showed the highest complement-fixing activity. It is composed of acidic polysaccharides [including glucuronoarabinoxylan (GAX), homogalacturonan (HGA), rhamnogalacturonan I (RG I)], neutral polysaccharides (4-galactan and arabinan), and some protein.

引用

页码：1193 / 1199

页数：7

共 29 条

[11] Regulation of cell proliferation, gene expression, production of cytokines, and cell cycle progression in primary human T lymphocytes by piperlactam S isolated from Piper kadsura [J].