APC-mediated proteolysis of Ase 1 and the morphogenesis of the mitotic spindle

被引：219

作者：

Juang, YL

Huang, J

Peters, JM

McLaughlin, ME

Tai, CY

Pellman, D

机构：

[1] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DEPT PEDIAT ONCOL,BOSTON,MA 02115

[2] HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT PEDIAT HEMATOL,BOSTON,MA 02115

[3] HARVARD UNIV,SCH MED,DEPT CELL BIOL,BOSTON,MA 02115

来源：

SCIENCE | 1997年 / 275卷 / 5304期

关键词：

D O I：

10.1126/science.275.5304.1311

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The molecular mechanisms that link cell-cycle controls to the mitotic apparatus are poorly understood. A component of the Saccharomyces cerevisiae spindle, Ase1, was observed to undergo cell cycle-specific degradation mediated by the cyclosome, or anaphase promoting complex (APC). Ase1 was degraded when cells exited from mitosis and entered G(1). Inappropriate expression of stable Ase1 during G(1) produced a spindle defect that is sensed by the spindle assembly checkpoint. In addition, loss of ASE1 function destabilized telophase spindles, and expression of a nondegradable Ase1 mutant delayed spindle disassembly. APC-mediated proteolysis therefore appears to regulate both spindle assembly and disassembly.

引用

页码：1311 / 1314

页数：4

共 34 条

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