Immuno-Raman microspectroscopy:: In situ detection of antigens in tissue specimens by surface-enhanced Raman scattering

被引：101

作者：

Schluecker, S. ^{[1
]}

Kuestner, B.

Punge, A.

Bonfig, R.

Marx, A.

Stroebel, P.

机构：

[1] Univ Wurzburg, Inst Phys Chem, D-97074 Wurzburg, Germany

[2] Univ Wurzburg, Urol Klin & Poliklin, D-97080 Wurzburg, Germany

[3] Univ Wurzburg, Inst Pathol, D-97080 Wurzburg, Germany

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2006年 / 37卷 / 07期

关键词：

prostate-specific antigen (PSA); antibody detection; tissue specimen; immunohistochemistry; Raman labels; surface-enhanced Raman scattering (SERS);

D O I：

10.1002/jrs.1534

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

We demonstrate the first realization of immuno-Raman microspectroscopy, a novel methodology in immunohistochemistry. The in situ detection of antigens in tissue specimens is achieved by surface-enhanced Raman scattering (SERS) from aromatic Raman labels covalently linked to the corresponding antibody. As an example of applications of this new Raman technique, we present the localization of prostate-specific antigen (PSA) in-epithelial tissue from patients with prostate carcinoma (PCA). Copyright (C) 2006 John Wiley & Sons, Ltd.

引用

页码：719 / 721

页数：3

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