High level transcription of a member of a repeated gene family confers dehydration tolerance to callus tissue of Craterostigma plantagineum

被引:104
作者
Furini, A [1 ]
Koncz, C [1 ]
Salamini, F [1 ]
Bartels, D [1 ]
机构
[1] MAX PLANCK INST ZUCHTUNGSFORSCH,D-50829 COLOGNE,GERMANY
关键词
abscisic acid; activation tagging; desiccation tolerance; regulatory RNA; resurrection plant;
D O I
10.1093/emboj/16.12.3599
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An experimental system has been developed which allows the identification of intermediates in the abscisic acid (ABA) signal transduction pathway leading to desiccation tolerance in plants. Desiccation tolerance in callus of the resurrection plant Craterostigma plantagineum is mediated via the plant hormone ABA, which induces the expression of gene products related to desiccation tolerance. Based on T-DNA activation tagging, a gene (CDT-1) was isolated which encodes a signalling molecule in the ABA transduction pathway. Constitutive overexpression of CDT-1 leads to desiccation tolerance in the absence of ABA and to the constitutive expression of characteristic transcripts. CDT-1 represents a novel gene with unusual features in its primary sequence. The CDT-1 gene resembles in several features SINE retrotransposons. Mechanisms by which CDT-1 activates the pathway could be via a regulatory RNA or via a short polypeptide.
引用
收藏
页码:3599 / 3608
页数:10
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