Ryanodine receptor point mutations confer diamide insecticide resistance in tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae)

被引：151

作者：

Roditakis, Emmanouil ^{[1
]}

Steinbach, Denise ^{[2
,3
]}

Moritz, Gerald ^{[1
,3
]}

Vasakis, Emmanouil ^{[1
]}

Stavrakaki, Marianna ^{[1
]}

Ilias, Aris

Garcia-Vidal, Lidia ^{[4
]}

del Rosario Martinez-Aguirre, Maria ^{[4
]}

Bielza, Pablo ^{[4
]}

Morou, Evangelia ^{[5
]}

Silva, Jefferson E. ^{[6
]}

Silva, Wellington M. ^{[6
]}

Siqueira, Herbert A. A. ^{[6
]}

Iqbal, Sofia ^{[7
]}

Troczka, Bartlomiej J. ^{[7
]}

Williamson, Martin S. ^{[7
]}

Bass, Chris ^{[8
]}

Tsagkarakou, Anastasia ^{[1
]}

Vontas, John ^{[5
,9
]}

Nauen, Ralf ^{[2
]}

机构：

[1] Hellen Agr Org Demeter, Inst Olive Tree Subtrop Crops & Viticulture, Iraklion, Crete, Greece

[2] Bayer CropSci, R&D Pest Control, Monheim, Germany

[3] Martin Luther Univ Halle Wittenberg, Dept Biol, Halle, Germany

[4] Univ Politecn Cartagena, Dept Prod Vegetal, Cartagena, Spain

[5] Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, Iraklion, Greece

[6] Univ Fed Rural Pernambuco UFRPE, Dept Agron, Recife, PE, Brazil

[7] Rothamsted Res, Harpenden, Herts, England

[8] Univ Exeter, Coll Life & Environm Sci, Penryn, England

[9] Agr Univ Athens, Dept Crop Sci, Athens, Greece

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2017年 / 80卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

Tuta absoluta; Diamide resistance; Flubendiamide; Chlorantraniliprole; Ryanodine receptor; Target-site mutation; FIELD-EVOLVED RESISTANCE; DIAMONDBACK MOTH; PLUTELLA-XYLOSTELLA; MEYRICK LEPIDOPTERA; BRAZILIAN POPULATIONS; CHLORANTRANILIPROLE; MODE; SUSCEPTIBILITY; GENETICS; CHANNEL;

D O I：

10.1016/j.ibmb.2016.11.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insect ryanodine receptors (RyR) are the molecular target-site for the recently introduced diamide insecticides. Diamides are particularly active on Lepidoptera pests, including tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae). High levels of diamide resistance were recently described in some European populations of T absoluta, however, the mechanisms of resistance remained unknown. In this study the molecular basis of diamide resistance was investigated in a diamide resistant strain from Italy (IT-GELA-SD4), and additional resistant field populations collected in Greece, Spain and Brazil. The genetics of resistance was investigated by reciprocally crossing strain IT-GELA-SD4 with a susceptible strain and revealed an autosomal incompletely recessive mode of inheritance. To investigate the possible role of target-site mutations as known from diamondback moth (Plutella xylostella), we sequenced respective domains of the RyR gene of T absoluta. Genotyping of individuals of IT-GELA-SD4 and field-collected strains showing different levels of diamide resistance revealed the presence of G4903E and I4746M RyR target-site mutations. These amino acid substitutions correspond to those recently described for diamide resistant diamondback moth, i.e. G4946E and I4790M. We also detected two novel mutations, G4903V and I4746T, in some of the resistant T. absoluta strains. Radioligand binding studies with thoracic membrane preparations of the IT-GELA-SD4 strain provided functional evidence that these mutations alter the affinity of the RyR to diamides. In combination with previous work on P. xylostella our study highlights the importance of position G4903 (G4946 in P. xylostella) of the insect RyR in defining sensitivity to diamides. The discovery of diamide resistance mutations in T absoluta populations of diverse geographic origin has serious implications for the efficacy of diamides under applied conditions. The implementation of appropriate resistance management strategies is strongly advised to delay the further spread of resistance. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：11 / 20

页数：10

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[1] A method of computing the effectiveness of an insecticide [J].