Solid phase extraction of clenbuterol from plasma using immunoaffinity followed by HPLC

被引：31

作者：

Rashid, BA

Kwasowski, P

Stevenson, D ^{[1
]}

机构：

[1] Univ Surrey, Sch Biol Sci, Robens Analyt Ctr, Guildford GU2 5XH, Surrey, England

[2] Univ Surrey, Sch Biol Sci, Clifmar Associates, Guildford GU2 5XH, Surrey, England

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1999年 / 21卷 / 03期

关键词：

clenbuterol; immuno-extraction; immunoaffinity; HPLC;

D O I：

10.1016/S0731-7085(99)00165-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An immune-extraction column for clenbuterol has been prepared. Optimum conditions for the selective retention and elution of clenbuterol have been developed, based on a modification of our earlier work on morphine, chlortoluron and isoproturon. Clenbuterol could be retained on the immune-column then eluted in one x one mi fraction using 50% methanol in phosphate buffered saline pH 9. On columns containing antisera (but not to clenbuterol) the clenbuterol was removed in the washing step. HPLC-UV determination gave clean traces. Day-to-day reproducibility was improved by precipitating the plasma proteins with acetonitrile. (C) 1999 Elsevier Science B.V. All rights reserved.

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页码：635 / 639

页数：5

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