A spectrophotometric method for assay of tannase using rhodanine

被引：242

作者：

Sharma, S

Bhat, TK ^{[1
]}

Dawra, RK

机构：

[1] Indian Vet Res Inst, Reg Stn, Palampur 176061, Himachal Prades, India

[2] HPKV, Coll Basic Sci, Dept Chem & Biochem, Palampur, Himachal Prades, India

来源：

ANALYTICAL BIOCHEMISTRY | 2000年 / 279卷 / 01期

关键词：

D O I：

10.1006/abio.1999.4405

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible. (C) 2000 Academic Press.

引用

页码：85 / 89

页数：5

共 26 条

[1] [Anonymous], HAKKO KOGAKU ZASSHI, DOI DOI 10.4061/2011/823619
[2] PURIFICATION AND SOME PROPERTIES OF YEAST TANNASE
AOKI, K
SHINKE, R
NISHIRA, H
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1976, 40 (01): : 79 - 85
[3] Tannin acyl hydrolase (EC 3.1.1.20) activity of Aspergillus, Penicillium, Fusarium and Trichoderma
Bajpai, B
Patil, S
[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1996, 12 (03) : 217 - 220
[4] PRODUCTION, PURIFICATION AND CHARACTERIZATION OF A TANNASE FROM ASPERGILLUS-NIGER LCF-8
BARTHOMEUF, C
REGERAT, F
POURRAT, H
[J]. JOURNAL OF FERMENTATION AND BIOENGINEERING, 1994, 77 (03): : 320 - 323
[5] Preliminary studies on tannin degradation by Aspergillus niger van Tieghem MTCC 2425
Bhat, TK
Makkar, HPS
Singh, B
[J]. LETTERS IN APPLIED MICROBIOLOGY, 1997, 25 (01) : 22 - 23
[6] Isolation of a tannin protein complex-degrading fungus from faeces of hill cattle
Bhat, TK
Makkar, HPS
Singh, B
[J]. LETTERS IN APPLIED MICROBIOLOGY, 1996, 22 (04) : 257 - 258
[7] BRIDGE P, 1996, METHODS MOL BIOL, V59
[8] BEVERAGE STABILIZATION THROUGH ENZYMATIC REMOVAL OF PHENOLICS
CANTARELLI, C
BRENNA, O
GIOVANELLI, G
ROSSI, M
[J]. FOOD BIOTECHNOLOGY, 1989, 3 (02) : 203 - 213
[9] Chatterjee R, 1996, BIOPROCESS ENG, V14, P159
[10] Coggon P., 1974, U.S. Patent, Patent No. [3812266, 3,812,266]

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