Human glial cell-line-derived neurotrophic factor: A structure-function analysis

被引：21

作者：

Chen, ZY

He, ZY

He, C

Lu, CL

Wu, XF

机构：

[1] Second Mil Med Univ, Dept Neurobiol, Shanghai 200433, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 268卷 / 03期

关键词：

D O I：

10.1006/bbrc.2000.2196

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glial cell-line-derived neurotrophic factor (GDNF) is a protein known to enhance the survival of dopaminergic and motor neurons, It has been shown to have therapeutic potential in the treatment of Parkinson's disease and other neurodegenerative diseases. GDNF gene was modified by deletion and insertion mutagenesis using PCR. methods. The various mutants were all highly expressed in Escherichia coli. The recombinant proteins were purified and their survival-promoting activities were determined by motor neurons. The result showed that the C-terminus was critical for structure stability of GDNF, and the alpha-helix, finger1 and finger2 regions were involved in receptor binding, while the N-terminus was not essential for the biological functions of GDNF. (C) 2000 Academic Press.

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页码：692 / 696

页数：5

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