FRET templated by G-quadruplex DNA: A specific ternary interaction using an original pair of donor/acceptor partners

被引：65

作者：

Allain, Clemence ^{[1
]}

Monchaud, David ^{[1
]}

Teulade-Fichou, Marie-Paule ^{[1
]}

机构：

[1] CNRS, UPR 285, Coll France, Lab Chim Interact Mol, F-75005 Paris, France

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 36期

关键词：

D O I：

10.1021/ja062193h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

G-quadruplex represents a suitable scaffold for FRET (fluorescence resonance energy transfer) since its two external quartets offer two well-defined binding sites for concomitant trapping of donor/acceptor partners. Combining selective G-quadruplex binders (macrocyclic bis(quinacridine) BOQ(1) or monomeric quinacridine MMQ(1), donor) with a highly fluorescent DNA probe (thiazole orange, acceptor), we designed a structure-specific FRET-system based on an unprecedented noncovalent ternary complex. This system could be potentially usable as a signature for quadruplex-DNA conformation in solution, but also might offer a unique means for observing cation and ligand binding influence on quadruplex topology.

引用

页码：11890 / 11893

页数：4

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