学术探索
学术期刊
学术作者
新闻热点
数据分析
智能评审

Comparison of Adipose-Derived and Bone Marrow Mesenchymal Stromal Cells in a Murine Model of Crohn's Disease

被引：39
作者：
Xie, Minghao [1 ,2 ]
Qin, Huabo [1 ,2 ]
Luo, Qianxin [1 ,2 ]
He, Xiaosheng [1 ,2 ]
He, Xiaowen [1 ,2 ]
Lan, Ping [1 ,2 ]
Lian, Lei [1 ,2 ]
机构：
[1] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Colorectal Surg, 26 Yuancun Erheng Rd, Guangzhou 510655, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Affiliated Hosp 6, Guangdong Prov Key Lab Colorectal & Pelv Floor Di, Guangzhou, Guangdong, Peoples R China
来源：
DIGESTIVE DISEASES AND SCIENCES | 2017年 / 62卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Mesenchymal stromal cells (MSCs); Crohn's disease (CD); Therapeutic effects; Intraperitoneal injection; STEM-CELLS; TISSUE; THERAPY; FISTULA; ADULT; TRANSPLANTATION; CULTURE; TRIAL; MSC;
D O I：
10.1007/s10620-016-4166-6
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
100201 [内科学];
摘要：
Mesenchymal stromal cells (MSCs) have been used in the treatment of Crohn's disease (CD) because of the immunomodulatory ability. The aim of this study was to investigate the therapeutic effect of adipose-derived MSCs (AD-MSCs) and to compare the therapeutic effect of AD-MSCs with that of bone marrow MSCs (BM-MSCs) in a murine model of CD. Murine colitis model of CD was created by trinitrobenzene sulfonic acid (TNBS). Twelve hours after treatment with TNBS, the mouse model was injected with MSCs intraperitoneally. Real-time polymerase chain reaction and immunohistochemistry staining were used to measure the expression levels of inflammatory cytokines in colonic tissues to investigate the therapeutic effect of AD-MSCs. The ten-day survival was recorded after infusion of MSCs. Intraperitoneal injection of MSCs alleviated the clinical and histopathologic severity of intestinal inflammation, and improved the survival of the TNBS-induced mouse model of CD. AD-MSCs could effectively increase the expression of interleukin-10 and reduce the secretion of pro-inflammatory cytokines including tumor necrosis factor-alpha, interleukin-12, and vascular endothelial growth factor. The mucosal injury was repaired by AD-MSCs. These effects were comparable between AD-MSCs and BM-MSCs. The therapeutic effect appears similar between AD-MSCs and BM-MSCs in treating CD. AD-MSCs may be a potential alternative of cell-based therapy for CD.
引用
收藏
页码：115 / 123
页数：9
相关论文
共 48 条
[1]
Adipose-derived stromal cells (ASCs) [J].
Bassi, Giulio ;
Pacelli, Luciano ;
Carusone, Roberta ;
Zanoncello, Jasmina ;
Krampera, Mauro .
TRANSFUSION AND APHERESIS SCIENCE, 2012, 47 (02) :193-198
[2]
Mesenchymal Stromal Cells A Novel Treatment Modality for Tissue Repair [J].
Bernardo, Maria Ester ;
Locatelli, Franco ;
Fibbe, Willem E. .
HEMATOPOIETIC STEM CELLS VII, 2009, 1176 :101-117
[3]
The immunological and genetic basis of inflammatory bowel disease [J].
Bouma, G ;
Strober, W .
NATURE REVIEWS IMMUNOLOGY, 2003, 3 (07) :521-533
[4]
Concise review: Mesenchymal stem cells: Their phenotype, differentiation capacity, immunological features, and potential for homing [J].
Chamberlain, Giselle ;
Fox, James ;
Ashton, Brian ;
Middleton, Jim .
STEM CELLS, 2007, 25 (11) :2739-2749
[5]
Long-Term Results of Adipose-Derived Stem Cell Therapy for the Treatment of Crohn's Fistula [J].
Cho, Yong Beom ;
Park, Kyu Joo ;
Yoon, Sang Nam ;
Song, Kee Ho ;
Kim, Do Sun ;
Jung, Sang Hun ;
Kim, Mihyung ;
Jeong, Hee Young ;
Yu, Chang Sik .
STEM CELLS TRANSLATIONAL MEDICINE, 2015, 4 (05) :532-537
[6]
Autologous bone marrow-derived mesenchymal stromal cells in the treatment of fistulising Crohn's disease [J].
Ciccocioppo, Rachele ;
Bernardo, Maria Ester ;
Sgarella, Adele ;
Maccario, Rita ;
Avanzini, Maria Antonietta ;
Ubezio, Cristina ;
Minelli, Antonella ;
Alvisi, Costanza ;
Vanoli, Alessandro ;
Calliada, Fabrizio ;
Dionigi, Paolo ;
Perotti, Cesare ;
Locatelli, Franco ;
Corazza, Gino Roberto .
GUT, 2011, 60 (06) :788-798
[7]
Crohn BB, 2000, MT SINAI J MED, V67, P263
[8]
Comparison of in vitro chondrogenic potential of human mesenchymal stem cells derived from bone marrow and adipose tissue [J].
Danisovic, L'ubos ;
Varga, Ivan ;
Polak, Stefan ;
Ulicna, Marcela ;
Hlavackova, Livia ;
Bohmer, Daniel ;
Vojtassak, Jan .
GENERAL PHYSIOLOGY AND BIOPHYSICS, 2009, 28 (01) :56-62
[9]
Comparison of multi-lineage cells from human adipose tissue and bone marrow [J].
De Ugarte, DA ;
Morizono, K ;
Elbarbary, A ;
Alfonso, Z ;
Zuk, PA ;
Zhu, M ;
Dragoo, JL ;
Ashjian, P ;
Thomas, B ;
Benhaim, P ;
Chen, I ;
Fraser, J ;
Hedrick, MH .
CELLS TISSUES ORGANS, 2003, 174 (03) :101-109
[10]
Pretreatment with Interferon-γ Enhances the Therapeutic Activity of Mesenchymal Stromal Cells in Animal Models of Colitis [J].
Duijvestein, Marjolijn ;
Wildenberg, Manon E. ;
Welling, Mick M. ;
Hennink, Simone ;
Molendijk, Ilse ;
van Zuylen, Vanessa L. ;
Bosse, Tjalling ;
Vos, Anne Christine W. ;
de Jonge-Muller, Eveline S. M. ;
Roelofs, Helene ;
van der Weerd, Louise ;
Verspaget, Hein W. ;
Fibbe, Willem E. ;
Velde, Anje A. Te ;
van den Brink, Gijs R. ;
Hommes, Daniel W. .
STEM CELLS, 2011, 29 (10) :1549-1558
12345