Investigation of biological activity of polar extracts isolated from Phlomis crinita Cav ssp mauritanica Munby

被引:13
作者
Amor, Ilef Limem-Ben [1 ,2 ]
Skandrani, Ines [1 ,2 ]
Boubaker, Jihed [1 ,2 ]
Sghaier, Mohamed Ben [1 ,2 ]
Neffati, Aicha [1 ,2 ]
Bhouri, Wissem [1 ,2 ]
Bouhlel, Ines [1 ,2 ]
Chouchane, Nabil [1 ,2 ]
Kilani, Soumaya [1 ,2 ]
Guedon, Emmanuel [3 ]
Ghoul, Mohamed [4 ]
Ghedira, Kamel [2 ]
Chekir-Ghedira, Leila [1 ]
机构
[1] Fac Med Dent, Cellular & Mol Biol Lab, Monastir 5019, Tunisia
[2] Fac Pharm, Unity Pharmacognosy Mol Biol, Monastir, Tunisia
[3] Inst Natl Polytech Lorraine, Lab Alimentary & Biotechnol Proc Engn, Vandoeuvre Les Nancy, France
[4] Inst Natl Polytech Lorraine, Bioproc & Biocatalysis Lab, Vandoeuvre Les Nancy, France
关键词
Phlomis crinita Cav. ssp mauritanica Munby; Antibacterial effect; Antiradical activity; SOS chromotest; Antiulcerogenic activity; ANTIOXIDANT ACTIVITY; MEDICINAL-PLANTS; PHENYLETHANOID GLYCOSIDE; ANTIBACTERIAL ACTIVITY; ANTIMICROBIAL ACTIVITY; SOS CHROMOTEST; STEM BARK; FLAVONOIDS; CONSTITUENTS; COUMARINS;
D O I
10.1080/01480540802416265
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The lyophilized infusion, the methanol, the ethyl acetate, and the total oligomer flavonoid (TOF)-enriched extracts prepared from the dried leaves of Phlomis crinita Cav. ssp. mauritanica Munby were investigated for the contents of flavonoids, tannins, coumarines and steroids. Antibacterial activity was investigated toward five bacterial strains. An inhibitory effect was observed against Staphyllococcus aureus and Enterococcus feacalis, and the minimal inhibitory concentrations ranged from 2.5 to 5 mg/mL of extract. The tested extracts exhibit an important free radical scavenging activity toward the 1,1-diphenyl 2-picrylhydrazyl (DPPH) free radical; with IC50 values of 30.5, 6, 32, and 31.5 mu g/mL, respectively, in the presence of lyophilized infusion, the TOF, the methanol, and the ethyl acetate extracts. Genotoxic and antigenotoxic properties of the different extracts were studied by using the SOS chromotest with Escherichia coli PQ37. The lyophilized infusion and TOF extracts obtained from P crinita ssp. mauritanica showed no genotoxicity, whereas methanol and ethyl acetate extracts are considered as marginally genotoxic. On the other hand, we showed that each extract inhibited the mutagenicity induced by aflatoxin B1 (AFB1) (10 mu g/assay) and nifuroxazide (NF) (10 mu g/assay). The ethyl acetate extract showed the strongest level of protection toward the genotoxicity induced by both directly and indirectly genotoxic NF and AFB1. These tests proved that the lyophilized infusion possesses an antiradical activity likewise, it showed no genotoxic effect; that is why we choose this extract to assess its antiulcerogenic activity by using an ethanol-induced ulcerogenesis model in the rat. This test demonstrates that 300 mg/kg of a P. crinita ssp. mauritanica lyophilized infusion was more effective than the reference compound, cimetidine.
引用
收藏
页码:38 / 46
页数:9
相关论文
共 40 条
[1]   Antibacterial activity of tannin constituents from Phaseolus vulgaris, Fagoypyrum esculentum, Corylus avellana and Juglans nigra [J].
Amarowicz, Ryszard ;
Dykes, Gary A. ;
Pegg, Ronald B. .
FITOTERAPIA, 2008, 79 (03) :217-219
[2]   Antioxidant activity of galloyl quinic derivatives isolated from P-lentiscus leaves [J].
Baratto, MC ;
Tattini, M ;
Galardi, C ;
Pinelli, P ;
Romani, A ;
Visioli, F ;
Basosi, R ;
Pogni, R .
FREE RADICAL RESEARCH, 2003, 37 (04) :405-412
[3]   Screening of antimutagenicity via antioxidant activity in different extracts from the leaves of Acacia salicina from the center of Tunisia [J].
Bouhlel, Ines ;
Ben Mansour, Hedi ;
Limem, Ilef ;
Ben Sghaier, Mohamed ;
Mahmoud, Amor ;
Ben Chibani, Jemni ;
Ghedira, Kamel ;
Chekir-Ghedira, Leila .
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2007, 23 (01) :56-63
[4]   Flavonoids from Phlomis nissolii [J].
Bucar, F ;
Ninov, S ;
Ionkova, I ;
Kartnig, T ;
Schubert-Zsilavecz, M ;
Asenov, I ;
Konuklugil, B .
PHYTOCHEMISTRY, 1998, 48 (03) :573-575
[5]   Inhibition of bacterial mutagenesis by Citrus flavonoids [J].
Calomme, M ;
Pieters, L ;
Vlietinck, A ;
VandenBerghe, D .
PLANTA MEDICA, 1996, 62 (03) :222-226
[6]  
Choi J S, 1994, Arch Pharm Res, V17, P71, DOI 10.1007/BF02974226
[7]   Antimicrobial evaluation of coumarins and flavonoids from the stems of Daphne gnidium L. [J].
Cottiglia, F ;
Loy, G ;
Garau, D ;
Floris, C ;
Casu, M ;
Pompei, R ;
Bonsignore, L .
PHYTOMEDICINE, 2001, 8 (04) :302-305
[8]   Essential oil of Phlomis lanata growing in Greece:: Chemical composition and antimicrobial activity [J].
Couladis, M ;
Tanimanidis, A ;
Tzakou, O ;
Chinou, IB ;
Harvala, C .
PLANTA MEDICA, 2000, 66 (07) :670-672
[9]   Antimicrobial activity of flavonoids [J].
Cushnie, TPT ;
Lamb, AJ .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2005, 26 (05) :343-356
[10]   Evaluation of mutagenic activity in an extract of pepper tree stem bark (Schinus terebinthifolius Raddi) [J].
de Carvalho, MCRD ;
Barca, FNTV ;
Agnez-Lima, LF ;
de Medeiros, SRB .
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2003, 42 (03) :185-191