A facile method for high-throughput co-expression of protein pairs

被引：89

作者：

Alexandrov, A

Vignali, M

LaCount, DJ

Quartley, E

de Vries, C

De Rosa, D

Babulski, J

Mitchell, SF

Schoenfeld, LW

Fields, S

Hol, WG

Dumont, ME

Phizicky, EM

Grayhack, EJ ^{[1
]}

机构：

[1] Univ Rochester, Med Ctr, Ctr Human Genet & Mol Pediat Dis, Rochester, NY 14642 USA

[2] Univ Rochester, Med Ctr, Struct Genom Pathogen Protozoa Consortium, Rochester, NY 14642 USA

[3] Univ Rochester, Med Ctr, Dept Biochem & Biophys, Rochester, NY 14642 USA

[4] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA

[5] Univ Washington, Dept Med, Seattle, WA 98195 USA

[6] Univ Washington, Howard Hughes Med Inst, Seattle, WA 98195 USA

[7] Univ Washington, Dept Biochem, Seattle, WA 98195 USA

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2004年 / 3卷 / 09期

关键词：

D O I：

10.1074/mcp.T400008-MCP200

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a method to co-express protein pairs from collections of otherwise identical Escherichia coli plasmids expressing different ORFs by incorporating a 61-nucleotide sequence ( LINK) into the plasmid to allow generation of tandem plasmids. Tandem plasmids are formed in a ligation-independent manner, propagate efficiently, and produce protein pairs in high quantities. This greatly facilitates co-expression for structural genomics projects that produce thousands of clones bearing identical origins and antibiotic markers.

引用

页码：934 / 938

页数：5