Attomole microarray detection of MicroRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions

被引：384

作者：

Fang, Shiping ^{[1
]}

Lee, Hye Jin ^{[1
]}

Wark, Alastair W. ^{[1
]}

Corn, Robert M. ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 43期

关键词：

D O I：

10.1021/ja065223p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Multiple microRNAs (miRNAs) are detected in a microarray format using a novel approach that combines a surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The surface reaction of poly(A) polymerase creates poly(A) tails on miRNAs hybridized onto locked nucleic acid (LNA) microarrays. DNA-modified nanoparticles are then adsorbed onto the poly(A) tails and detected with SPRI. This ultrasensitive nanoparticle-amplified SPRI methodology can be used for miRNA profiling at attomole levels. Copyright © 2006 American Chemical Society.

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页码：14044 / 14046

页数：3

相关论文

共 14 条

[1] Probing microRNAs with microarrays: Tissue specificity and functional inference [J].

Babak, T ;

Zhang, W ;

Morris, Q ;

Blencowe, BJ ;

Hughes, TR .

RNA, 2004, 10 (11) :1813-1819

[2] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[3] Gene regulation by microRNAs [J].

Carthew, RW .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2006, 16 (02) :203-208

[4] A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA) [J].

Castoldi, M ;

Schmidt, S ;

Benes, V ;

Noerholm, M ;

Kulozik, AE ;

Hentze, MW ;

Muckenthaler, MU .

RNA, 2006, 12 (05) :913-920

[5] Real-time quantification of microRNAs by stem-loop RT-PCR [J].

Chen, CF ;

Ridzon, DA ;

Broomer, AJ ;

Zhou, ZH ;

Lee, DH ;

Nguyen, JT ;

Barbisin, M ;

Xu, NL ;

Mahuvakar, VR ;

Andersen, MR ;

Lao, KQ ;

Livak, KJ ;

Guegler, KJ .

NUCLEIC ACIDS RESEARCH, 2005, 33 (20) :e179.1-e179.9

[6] Detection of microRNAs using electrocatalytic nanoparticle tags [J].

Gao, ZQ ;

Yang, ZC .

ANALYTICAL CHEMISTRY, 2006, 78 (05) :1470-1477

[7] Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization [J].

He, L ;

Musick, MD ;

Nicewarner, SR ;

Salinas, FG ;

Benkovic, SJ ;

Natan, MJ ;

Keating, CD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (38) :9071-9077

[8] Creating advanced multifunctional biosensors with surface enzymatic transformations [J].

Lee, Hye Jin ;

Wark, Alastair W. ;

Corn, Robert M. .

LANGMUIR, 2006, 22 (12) :5241-5250

[9] Single-nucleotide polymorphism genotyping by nanoparticle-enhanced surface plasmon resonance imaging measurements of surface ligation reactions [J].

Li, Yuan ;

Wark, Alastair W. ;

Lee, Hye Jin ;

Corn, Robert M. .

ANALYTICAL CHEMISTRY, 2006, 78 (09) :3158-3164

[10] An oligonucleotide microarray for microRNA expression analysis based on labeling RNA with quantum dot and nanogold probe [J].

Liang, RQ ;

Li, W ;

Li, Y ;

Tan, CY ;

Li, JX ;

Jin, YX ;

Ruan, KC .

NUCLEIC ACIDS RESEARCH, 2005, 33 (02) :e17