Tiny telomere DNA

被引：72

作者：

Ren, JS

Qu, XG

Trent, JO

Chaires, JB

机构：

[1] Univ Mississippi, Med Ctr, Dept Biochem, Jackson, MS 39216 USA

[2] Univ Louisville, Dept Med, JG Brown Canc Ctr, Louisville, KY 40202 USA

来源：

NUCLEIC ACIDS RESEARCH | 2002年 / 30卷 / 11期

关键词：

D O I：

10.1093/nar/30.11.2307

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the design, synthesis and biophysical characterization of a novel DNA construct in which a folded quadruplex structure is joined to a standard double helix. Circular dichroism, gel electrophoresis, three-dimensional UV melting and differential scanning calorimetry were all used to characterize the structure. Rigorous molecular dynamics simulations were used to build a plausible atomic-level structural model of the DNA construct. This novel DNA construct provides a model for the duplex-quadruplex junction region at the end of chromosomal DNA and offers a system for the study of structure-selective ligand binding.

引用

页码：2307 / 2315

页数：9

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