Confinement and detection of single molecules in submicrometer channels

被引：96

作者：

Lyon, WA ^{[1
]}

Nie, SM ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 16期

关键词：

D O I：

10.1021/ac9700742

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Detection channels with submicrometer dimensions (500-600 nn i.d.) have been produced directly on electrophoresis capillaries for confinement and detection of single molecules at room temperature. These restrictive channels markedly reduce the Brownian motion of low molecular weight analytes and allow detailed studies of single molecules in solution. With a confocal fluorescence microscope, single molecules have been observed for an extended period of similar to 60 ms, which is 50-100 times longer than the bulk diffusion time. These molecules have also been manipulated by an electrokinetic force and detected on-line with high signal-to-noise ratios.

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页码：3400 / 3405

页数：6

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