A metabonomic study of hepatitis B-induced liver cirrhosis and hepatocellular carcinoma by using RP-LC and HILIC coupled with mass spectrometry

被引：225

作者：

Yin, Peiyuan ^{[1
]}

Wan, Dafang ^{[2
]}

Zhao, Chunxia ^{[1
]}

Chen, Jing ^{[1
]}

Zhao, Xinjie ^{[1
]}

Wang, Wenzhao ^{[1
]}

Lu, Xin ^{[1
]}

Yang, Shengli ^{[1
,2
,3
]}

Gu, Jianren ^{[2
]}

Xu, Guowang ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Shanghai Canc Inst, Natl Lab Oncogenes & Related Genes, Shanghai 200032, Peoples R China

[3] Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai 200032, Peoples R China

来源：

MOLECULAR BIOSYSTEMS | 2009年 / 5卷 / 08期

基金：

中国国家自然科学基金;

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; HYDROPHILIC INTERACTION CHROMATOGRAPHY; MULTIVARIATE STATISTICAL-ANALYSIS; INSULIN-RESISTANT MICE; FARNESOID-X-RECEPTOR; SYSTEMS BIOLOGY; H-1-NMR SPECTROSCOPY; ACID-METABOLISM; RAT URINE; BILE-ACID;

D O I：

10.1039/b820224a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Liver cirrhosis and hepatocellular carcinoma (HCC) are fatal sequelaes of chronic hepatitis B in China. The sera from HCC and cirrhosis were profiled by rapid resolution liquid chromatography coupled with quadrupole time-of-flight (Q-TOF) mass spectrometry. Reversed-phased (RP) liquid chromatography and hydrophilic interaction chromatography (HILIC) were used for the data acquisition. The normalized and combined data were handled by chemometric analysis, and the combination proved to be effective and reliable for the orthogonal projection to latent structures (OPLS) analysis. Metabonomic profiles and the potential biomarkers were found based on the OPLS models. Shared and unique structure (SUS) plots were used for the evaluation of the potential biomarkers. Glycocholic acid, glycochenodeoxycholic acid, taurocholic acid and taurochenodesoxycholic acid were found to be potential biomarkers related to liver cirrhosis, while dihydrosphingosine and phytosphingosine were potential diagnostic biomarkers of HCC. The other identified metabolites were considered as common potential biomarkers for the two liver diseases. Correlation networks based on these metabolites were also built for the systemic understanding of these diseases and the possible biological implications are discussed. This metabonomic approach may provide insight into discovery and identification of new diagnostic biomarkers for liver cancer and associated diseases.

引用

页码：868 / 876

页数：9

共 45 条

[1]

A combined 1H-NMR spectroscopy- and mass spectrometry-based metabolomic study of the PPAR-α null mutant mouse defines profound systemic changes in metabolism linked to the metabolic syndrome [J].