Presence of acylated homoserine lactones (AHLs) and AHL-producing bacteria in meat and potential role of AHL in spoilage of meat

被引:105
作者
Bruhn, JB
Christensen, AB
Flodgaard, LR
Nielsen, KF
Larsen, TO
Givskov, M
Gram, L
机构
[1] Tech Univ Denmark, Danish Inst Fisheries Res, Dept Seafood Res, BioCentrum,DTU, DK-2800 Lyngby, Denmark
[2] Tech Univ Denmark, Ctr Biomed Microbiol, Sect Mol Microbiol, DK-2800 Lyngby, Denmark
[3] Tech Univ Denmark, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark
关键词
D O I
10.1128/AEM.70.7.4293-4302.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Quorum-sensing (QS) signals (N-acyl homoserine lactones [AHLs]) were extracted and detected from five commercially produced vacuum-packed meat samples. Ninety-six AHL-producing bacteria were isolated, and 92 were identified as Enterobacteriaceae. Hafnia alvei was the most commonly identified AHL-producing bacterium. Thin-layer chromatographic profiles of supernatants from six H. alvei isolates and of extracts from spoiling meat revealed that the major AHL species had an R-f value and shape similar to N-3-oxo-hexanoyl homoserine lactone (OHHL). Liquid chromatography-mass spectrometry (MS) (high-resolution MS) analysis confirmed the presence of OHHL in pure cultures of H. alvei. Vacuum-packed meat spoiled at the same rate when inoculated with the H. alvei wild type compared to a corresponding AHL-lacking mutant. Addition of specific QS inhibitors to the AHL-producing H. alvei inoculated in meat or to naturally contaminated meat did not influence the spoilage of vacuum-packed meat. An extracellular protein of approximately 20 kDa produced by the H. alvei wild-type was not produced by the AHL-negative mutant but was restored in the mutant when complemented by OHHL, thus indicating that AHLs do have a regulatory role in H. alvei. Coinoculation of H. alvei wild-type with an AHL-deficient Serratia proteamaculans B5a, in which protease secretion is QS regulated, caused spoilage of liquid milk. By contrast, coinoculation of AHL-negative strains of H. alvei and S. proteamaculans B5a did not cause spoilage. In conclusion, AHL and AHL-producing bacteria are present in vacuum-packed meat during storage and spoilage, but AHL does not appear to influence the spoilage of this particular type of conserved meat. Our data indicate that AHL-producing H. alvei may induce food quality-relevant phenotypes in other bacterial species in the same environment. H. alvei may thus influence spoilage of food products in which Enterobacteriaceae participate in the spoilage process.
引用
收藏
页码:4293 / 4302
页数:10
相关论文
共 57 条
[1]   Quorum-sensing system and stationary-phase sigma factor (rpoS) of the onion pathogen Burkholderia cepacia genomovar I type strain, ATCC 25416 [J].
Aguilar, C ;
Bertani, I ;
Venturi, V .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (03) :1739-1747
[2]   Salmonella typhimurium encodes an SdiA homolog, a putative quorum sensor of the LuxR family, that regulates genes on the virulence plasmid [J].
Ahmer, BMM ;
van Reeuwijk, J ;
Timmers, CD ;
Valentine, PJ ;
Heffron, F .
JOURNAL OF BACTERIOLOGY, 1998, 180 (05) :1185-1193
[3]   A hierarchical quorum-sensing system in Yersinia pseudotuberculosis is involved in the regulation of motility and clumping [J].
Atkinson, S ;
Throup, JP ;
Stewart, GSAB ;
Williams, P .
MOLECULAR MICROBIOLOGY, 1999, 33 (06) :1267-1277
[4]   N-(3-OXOHEXANOYL)-L-HOMOSERINE LACTONE REGULATES CARBAPENEM ANTIBIOTIC PRODUCTION IN ERWINIA-CAROTOVORA [J].
BAINTON, NJ ;
STEAD, P ;
CHHABRA, SR ;
BYCROFT, BW ;
SALMOND, GPC ;
STEWART, GSAB ;
WILLIAMS, P .
BIOCHEMICAL JOURNAL, 1992, 288 :997-1004
[5]  
Barrow GI., 1993, COWAN STEELS MANUAL, V3th
[6]   EFFECT OF INITIAL INTERNAL TEMPERATURE AND STORAGE TEMPERATURE ON MICROBIAL-FLORA OF VACUUM PACKAGED BEEF [J].
BEEBE, SD ;
VANDERZANT, C ;
HANNA, MO ;
CARPENTER, ZL ;
SMITH, GC .
JOURNAL OF MILK AND FOOD TECHNOLOGY, 1976, 39 (09) :600-605
[8]   Bacterial spoilage of meat and cured meat products [J].
Borch, E ;
KantMuermans, ML ;
Blixt, Y .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1996, 33 (01) :103-120
[9]   Production of acylated homoserine lactones by different serotypes of Vibrio anguillarum both in culture and during infection of rainbow trout [J].
Buch, C ;
Sigh, J ;
Nielsen, J ;
Larsen, JL ;
Gram, L .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2003, 26 (03) :338-349
[10]   Nonenzymatic turnover of an Erwinia carotovora quorum-sensing signaling molecule [J].
Byers, JT ;
Lucas, C ;
Salmond, GPC ;
Welch, M .
JOURNAL OF BACTERIOLOGY, 2002, 184 (04) :1163-1171