The promoter for the ribosomal RNA genes of Leishmania chagasi

被引：31

作者：

Gay, LS

Wilson, ME

Donelson, JE

机构：

[1] UNIV IOWA,DEPT BIOCHEM,IOWA CITY,IA 52242

[2] HOWARD HUGHES MED INST,IOWA CITY,IA 52242

[3] UNIV IOWA,DEPT MED & MICROBIOL,IOWA CITY,IA 52242

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1996年 / 77卷 / 02期

关键词：

protozoa; Leishmania chagasi; promoter; ribosomal RNA genes; RNA polymerase I; transient transfections; luciferase;

D O I：

10.1016/0166-6851(96)02594-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A promoter for the rRNA genes of Leishmania chagasi was found to be located about 1 kb upstream of the 18S rRNA coding region and immediately downstream of 64 bp tandem repeats. Its approximate boundaries and corresponding transcription start site were determined by transient transfections and primer extension assays. This promoter for RNA polymerase I has differing activities when transfected into various Leishmania species and no activity in Trypanosoma cruzi. Its sequence has no obvious similarities with other known rRNA promoters in Trypanosomatids. Depending on the species, this promoter can be used to increase expression of a protein from a plasmid in Leishmania by as much as 45-fold over that from a plasmid lacking a promoter.

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收藏

页码：193 / 200

页数：8

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