THE TRYPANOSOMA-CRUZI RIBOSOMAL RNA-ENCODING GENE - ANALYSIS OF PROMOTER AND UPSTREAM INTERGENIC SPACER SEQUENCES

被引：42

作者：

DIETRICH, P ^{[1
]}

SOARES, MB ^{[1
]}

AFFONSO, MHT ^{[1
]}

FLOETERWINTER, LM ^{[1
]}

机构：

[1] UNIV SAO PAULO, INST CIENCIAS BIOMED, DEPT PARASITOL, SAO PAULO, BRAZIL

来源：

GENE | 1993年 / 125卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

RIBOSOMAL DNA ORGANIZATION; RECOMBINANT DNA; RNA POLYMERASE-I PROMOTER; REPETITIVE SEQUENCES;

D O I：

10.1016/0378-1119(93)90753-P

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The transcription start point (tsp) of the ribosomal RNA(rRNA)-encoding gene of Trypanosoma cruzi was mapped at 1550 bp upstream from the 18S rRNA coding sequence. The + 1 nucleotide (tsp) was determined to be a guanosine. As described for other eukaryotes, no consensus sequence was found when the putative promoter sequence (- 200 to + 50) was compared with that described for Trypanosoma brucei and Crithidia fasciculata. However, a repeated element was found in the upstream intergenic spacer sequence (IGS) of T. cruzi. Motifs, present in this element, exhibit significant homology to the T. cruzi promoter sequence. Furthermore, the same motifs could be found, in a similar sequence organization, within the T. brucei promoter region. Therefore, the data described in this paper strongly indicate that the IGS rDNA (DNA coding for rRNA) organization in trypanosomatids appears similar to that found in higher eukaryotes.

引用

页码：103 / 107

页数：5

共 24 条

[1]

BIRBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] LEISHMANIA-TARENTOLAE TAXONOMIC RELATEDNESS INFERRED FROM PHYLOGENETIC ANALYSIS OF THE SMALL SUBUNIT RIBOSOMAL-RNA GENE [J].

BRIONES, MRS ;

NELSON, K ;

BEVERLEY, SM ;

AFFONSO, HT ;

CAMARGO, EP ;

FLOETERWINTER, LM .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 53 (1-2) :121-128

[3]

CamargO E. P., 1964, Revista do Instituto de Medicina Tropical de Sao Paulo, V6, P93

[4] PRECISE IDENTIFICATION OF CLEAVAGE SITES INVOLVED IN THE UNUSUAL PROCESSING OF TRYPANOSOME RIBOSOMAL-RNA [J].

CAMPBELL, DA ;

KUBO, K ;

CLARK, CG ;

BOOTHROYD, JC .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (01) :113-124

[5] RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS IN THE RIBOSOMAL GENE SPACERS OF TRYPANOSOMA-CRUZI AND TRYPANOSOMA-CONORHINI [J].

DIETRICH, P ;

DUSSAN, MD ;

FLOETERWINTER, LM ;

AFFONSO, MHT ;

CAMARGO, EP ;

SOARES, MB .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 42 (01) :13-19

[6] MULTIPLE REPEATED UNITS IN DROSOPHILA-MELANOGASTER RIBOSOMAL DNA SPACER STIMULATE RIBOSOMAL-RNA PRECURSOR TRANSCRIPTION [J].

GRIMALDI, G ;

DINOCERA, PP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (15) :5502-5506

[7] IDENTIFICATION AND SEQUENCE-ANALYSIS OF THE RIBOSOMAL DNA PROMOTER REGION OF CRITHIDIA-FASCICULATA [J].

GRONDAL, EJM ;

EVERS, R ;

CORNELISSEN, AWCA .

NUCLEIC ACIDS RESEARCH, 1990, 18 (06) :1333-1338

[8] PRIMARY STRUCTURE OF TRYPANOSOMA-CRUZI SMALL-SUBUNIT RIBOSOMAL-RNA CODING REGION - COMPARISON WITH OTHER TRYPANOSOMATIDS [J].

HERNANDEZ, R ;

RIOS, P ;

VALDES, AM ;

PINERO, D .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 41 (02) :207-212

[9]

HU NT, 1982, GENE, V17, P271

[10] ENHANCER-LIKE PROPERTIES OF THE 60/81 BP ELEMENTS IN THE RIBOSOMAL GENE SPACER OF XENOPUS-LAEVIS [J].

LABHART, P ;

REEDER, RH .

CELL, 1984, 37 (01) :285-289

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