Transglutaminase reactivity of human involucrin

被引:5
作者
Lambert, A
Ekambaram, M
Robinson, N
Eckert, RL
机构
[1] Case Western Reserve Univ, Sch Med, Dept Physiol & Biophys, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Sch Med, Dept Physiol & Biophys, Cleveland, OH 44106 USA
[3] Case Western Reserve Univ, Sch Med, Dept Dermatol, Cleveland, OH 44106 USA
[4] Case Western Reserve Univ, Sch Med, Dept Reprod Biol, Cleveland, OH 44106 USA
[5] Case Western Reserve Univ, Sch Med, Dept Oncol, Cleveland, OH 44106 USA
来源
SKIN PHARMACOLOGY AND APPLIED SKIN PHYSIOLOGY | 2000年 / 13卷 / 01期
关键词
transglutaminase; cornified envelope; keratinocyte; differentiation; cross-linking; isopeptide bond; apoptosis;
D O I
10.1159/000029905
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Human involucrin (hINV) is assembled into cornified structures via formation of transglutaminase (TG)-dependent interprotein epsilon-(gamma-glutamyl)lysine bonds. The hINV sequence includes 150 glutamine residues that could function as potential sites of cross-link formation. The present studies were designed to evaluate the extent to which hINV can function as a TG substrate under optimal conditions and in the absence of other substrates, Incubation of hINV with TG results in formation of 4-5 isopeptide bonds per hINV molecule. When the small amine donor C-14-putrescine is included in the reaction, 48 Q residues are tabled, Isotope distribution and sequence analysis suggests that the C-14-putrescine-labeled sites are located throughout the protein. Our present results show that many hINV Q residues can be utilized for cross-link formation, and that hINV can be cross-linked at very high cross-link densities. These results suggest that, in vivo, factors other than hINV structure limit the number of residues used for cross-link formation. Copyright (C) 2000 S. Karger AG, Basel.
引用
收藏
页码:17 / 30
页数:14
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