Comparisons of porcine hepatocyte spheroids and single hepatocytes in the non-woven fabric bioartificial liver module

被引：34

作者：

Naruse, K ^{[1
]}

Sakai, Y ^{[1
]}

Nagashima, I ^{[1
]}

Jiang, GX ^{[1
]}

Suzuki, M ^{[1
]}

Muto, T ^{[1
]}

机构：

[1] UNIV TOKYO, INST IND SCI, MINATO KU, TOKYO 106, JAPAN

来源：

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS | 1996年 / 19卷 / 10期

关键词：

bioartificial liver; non-woven fabric bioreactor; porcine hepatocytes; hepatocyte spheroids; suspension culture;

D O I：

10.1177/039139889601901008

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We previously developed a new bioreactor of the bioartificial liver composed of nonwoven fabric. We have also experimented with hepatocyte spheroids, with the aim of improving the efficiency of this NWF bioreactor. In this study we compared the efficiencies of NWF bioreactors employing hepatocyte spheroids versus single hepatocytes. Hepatocytes were isolated from a whole pig liver by Seglen's method. 1.0 x 10(10) single hepatocytes were immobilized in the NWF bioreactor. Another 1.0 x 10(10) hepatocytes were allowed to form spheroids by 24 hr suspension culture in a 4-L culture vessel, before being immobilized in the bioreactor. Hepatocyte spheroids were found to be functionally superior, on a per-cell basis, to single hepatocytes in the NWF bioreactor. However, the NWF bioreactor employing hepatocyte spheroids exhibited lower efficiency than that employing single hepatocytes, because the fetal number of the hepatocytes had decreased during the 24 hr suspension culture.

引用

页码：605 / 609

页数：5

共 8 条

[1]

MARSH WH, 1965, CLIN CHEM, V11, P624

[2] DETERMINATION OF AMMONIA IN- WHOLE BLOOD BY A DIRECT COLORIMETRIC METHOD [J].