Investigation by NMR spectroscopy and molecular modeling of the conformations of some modified disaccharide antigens for Shigella dysenteriae type 1

被引：7

作者：

Coxon, B ^{[1
]}

Sari, N ^{[1
]}

Mulard, LA ^{[1
]}

Kovac, P ^{[1
]}

Pozsgay, V ^{[1
]}

Glaudemans, CPJ ^{[1
]}

机构：

[1] NIDDKD,MED CHEM LAB,NIH,BETHESDA,MD 20892

来源：

JOURNAL OF CARBOHYDRATE CHEMISTRY | 1997年 / 16卷 / 06期

关键词：

D O I：

10.1080/07328309708006549

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The O-polysaccharide of Shigella dysenteriae type 1 is made up of multiple repeats of the linear tetrasaccharide 3)-alpha-L-Rhap-(1-->2)-alpha-D-Galp-(1-->3)-alpha-D-GlcpNAc-(1-->3)-alpha-L-Rhap-(1-->, for which the antigenic determinant for a murine monoclonal IgM antibody is the disaccharide alpha-L-Rhap-(1-->2)-alpha-D-Galp. This disaccharide and various analogs have been studied by 2D NOESY, POESY, and TOCSY NMR spectroscopy, in conjunction with proton spin-lattice relaxation rate measurements, restrained molecular mechanics, and restrained molecular dynamics with simulated annealing. It has been found that replacement of any single hydroxyl group in the determinant by a hydrogen atom, or replacement of any single hydroxyl group in the Gal residue by a fluorine atom has little if any influence on the conformation of the resulting derivatives.

引用

页码：927 / 946

页数：20

共 35 条

[1] IDENTIFICATION OF PROTEIN-MEDIATED INDIRECT NOE EFFECTS IN A DISACCHARIDE-FAB' COMPLEX BY TRANSFERRED ROESY [J].