Metals and the Integrity of a Biological Coating: The Cuticle of Mussel Byssus

被引：174

作者：

Holten-Andersen, Niels ^{[1
]}

Mates, Thomas E. ^{[2
]}

Toprak, Muhammet S. ^{[3
]}

Stucky, Galen D. ^{[3
]}

Zok, Frank W. ^{[2
]}

Waite, J. Herbert ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

来源：

LANGMUIR | 2009年 / 25卷 / 06期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

CROSS-LINKING; OOCYST WALL; PRECURSOR; PROTEINS; COLLAGEN;

D O I：

10.1021/la8027012

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The cuticle of mussel byssal threads is a robust natural coating that combines high extensibility with high stiffness and hardness. In this study, fluorescence microscopy and elemental analysis were exploited to show that the 3,4-dihydroxyphenyl-L-alanine (dopa) residues of mussel foot protein-1 colocalize with Fe and Ca distributions in the cuticle of Mytilus galloprovincialis mussel byssal threads. Chelated removal of Fe and Ca from the cuticle of intact threads resulted in a 50% reduction in cuticle hardness, and thin sections subjected to the same treatment showed a disruption of cuticle integrity. Dopa-metal complexes may provide significant interactions for the integrity of composite cuticles deformed under tension.

引用

页码：3323 / 3326

页数：4

共 37 条

[1] The extensible alloscutal cuticle of the tick, Ixodes ricinus [J].

Andersen, SA ;

Roepstorff, P .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2005, 35 (10) :1181-1188

[2] COORDINATION CHEMISTRY OF MICROBIAL IRON TRANSPORT COMPOUNDS .9. STABILITY-CONSTANTS FOR CATECHOL MODELS OF ENTEROBACTIN [J].

AVDEEF, A ;

SOFEN, SR ;

BREGANTE, TL ;

RAYMOND, KN .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1978, 100 (17) :5362-5370

[3] The coccidian oocyst: a tough nut to crack! [J].

Belli, Sabina I. ;

Smith, Nicholas C. ;

Ferguson, David J. P. .

TRENDS IN PARASITOLOGY, 2006, 22 (09) :416-423

[4] Roles of tyrosine-rich precursor glycoproteins and dityrosine- and 3,4-dihydroxyphenylalanine-mediated protein cross-linking in development of the oocyst wall in the coccidian parasite Eimeria maxima [J].

Belli, SI ;

Wallach, MG ;

Luxford, C ;

Davies, MJ ;

Smith, NC .

EUKARYOTIC CELL, 2003, 2 (03) :456-464

[5] Critical role of zinc in hardening of Nereis jaws [J].

Broomell, Chris C. ;

Mattoni, Mike A. ;

Zok, Frank W. ;

Waite, J. Herbert .

JOURNAL OF EXPERIMENTAL BIOLOGY, 2006, 209 (16) :3219-3225

[6]

COOK M, 1970, ADHES BIOL SYST

[7]

COULON J, 1987, ANN I OCEANOGR PARIS, V63, P89

[8] Extensible collagen in mussel byssus: A natural block copolymer [J].

Coyne, KJ ;

Qin, XX ;

Waite, JH .

SCIENCE, 1997, 277 (5333) :1830-1832

[9] Coating of jute with natural rubber [J].

Das, D ;

Datta, M ;

Chavan, RB ;

Datta, SK .

JOURNAL OF APPLIED POLYMER SCIENCE, 2005, 98 (01) :484-489

[10] Cohesive and adhesive properties of polycaprolactone/silica hybrid coatings on poly(methyl methacrylate) substrates [J].

Fabbri, P. ;

Singh, B. ;

Leterrier, Y. ;

Manson, J. -A. E. ;

Messori, M. ;

Pilati, F. .

SURFACE & COATINGS TECHNOLOGY, 2006, 200 (24) :6706-6712

← 1 2 3 4 →