MafT, a new member of the small Maf protein family in zebrafish

被引：51

作者：

Takagi, Y

Kobayashi, M

Li, L

Suzuki, T

Nishikawa, K

Yamamoto, M

机构：

[1] Univ Tsukuba, ERATO, JST, Ctr TARA, Tsukuba, Ibaraki 3058575, Japan

[2] Univ Tsukuba, Inst Basic Med Sci, Tsukuba, Ibaraki 3058575, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 320卷 / 01期

基金：

日本学术振兴会;

关键词：

diethylmaleate; heterodimer; luciferase assay; Nrf2; p45; Nfe2; phase; 2; induction; radiation hybrid mapping; small maf protein; transcription; zebrafish;

D O I：

10.1016/j.bbrc.2004.05.131

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small Maf proteins play critical roles on morphogenesis and homeostasis through associating with CNC proteins. To date, three small Maf proteins, MafF, MafG, and MafK, have been reported in vertebrates, which share redundant functions. In this study, we tried to identify and characterize small Maf proteins in zebrafish to elucidate their conservation and diversity in the fish kingdom. We identified homolog genes of MafG and MafK but not MafF in zebrafish, indicating the former two are conserved among vertebrates. In addition, a novel type of small Maf protein MalT was identified. MafT protein bound MARE sequence as a homodimer or heterodimers with zebrafish Nrf2 or p45 Nfe2. Co-overexpression of MafT and Nrf2 synergistically activated MARE-mediated gene expression in zebrafish embryos. These results indicated that MafT is a new member of small Maf proteins and involved in the Nrf2-dependent gene regulation in cellular defense system. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：62 / 69

页数：8

共 45 条

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