Metabolomics and Isotope Tracing

被引：724

作者：

Jang, Cholsoon ^{[1
,2
]}

Chen, Li ^{[1
,2
]}

Rabinowitz, Joshua D. ^{[1
,2
]}

机构：

[1] Princeton Univ, Lewis Sigler Inst Integrat Genom, Washington Rd, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Chem, Washington Rd, Princeton, NJ 08544 USA

来源：

CELL | 2018年 / 173卷 / 04期

基金：

美国国家卫生研究院;

关键词：

CELL LUNG-CANCER; MASS-SPECTROMETRY; NMR-SPECTROSCOPY; METABOLITE CONCENTRATIONS; LIQUID-CHROMATOGRAPHY; GLUTAMINE-METABOLISM; ESCHERICHIA-COLI; TUMOR-CELLS; PROTEIN; FLUX;

D O I：

10.1016/j.cell.2018.03.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.

引用

页码：822 / 837

页数：16

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