The immunological activity of Lycium barbarum polysaccharides liposome in vitro and adjuvanticity against PCV2 in vivo

被引：62

作者：

Bo, Ruonan ^{[1
]}

Zheng, Sisi ^{[1
]}

Xing, Jie ^{[1
]}

Luo, Li ^{[1
]}

Niu, Yale ^{[1
]}

Huang, Yee ^{[1
]}

Liu, Zhenguang ^{[1
]}

Hu, Yuanliang ^{[1
]}

Liu, Jiaguo ^{[1
]}

Wu, Yi ^{[1
]}

Wang, Deyun ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 85卷

基金：

中国国家自然科学基金;

关键词：

Lycium barbarum polysaccharides liposome; Immunological activity; Vaccine adjuvant; Immune responses; TOLL-LIKE RECEPTORS; REGULATORY T-CELLS; VACCINE ADJUVANTS; ASTRAGALUS POLYSACCHARIDE; IMMUNE-RESPONSE; DELIVERY; MACROPHAGES; NANOPARTICLES; IMPROVE;

D O I：

10.1016/j.ijbiomac.2015.12.089

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In previous researches, the results showed that Lycium barbarum polysaccharides (LBP) encapsulated with liposome could enhance the immune activity of LBP. Therefore, the present study was designed to investigate the effects of LBPL on spleen lymphocytes and macrophages of mice in vitro and evaluate the immunological adjuvant activity of PCV2 vaccine in vivo. The results showed that LBPL could significantly promote splenocyte proliferation synergistically with PHA or LPS, increase the ratio of CD4(+) to CD8(+) T cells and promote the cytokine secretion of macrophages; enhance PCV2-specific IgG antibody responses, promote Th1 cytokines (IFN-gamma and TNF-alpha) and Th2 cytokine (IL-4) secretion. The histomorphological observation of spleen demonstrated that LBPL as a vaccine adjuvant also has good improvement and stimulating effect on the immune organ. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：294 / 301

页数：8

共 43 条

[1] Toll-like receptors in the induction of the innate immune response
Aderem, A
Ulevitch, RJ
[J]. NATURE, 2000, 406 (6797) : 782 - 787
[2] Vaccine adjuvants revisited
Aguilar, J. C.
Rodriguez, E. G.
[J]. VACCINE, 2007, 25 (19) : 3752 - 3762
[3] A randomized, double-blind, placebo-controlled, clinical study of the general effects of a standardized Lycium barbarum (Goji) juice, GoChi™
Amagase, Harunobu
Nance, Dwight M.
[J]. JOURNAL OF ALTERNATIVE AND COMPLEMENTARY MEDICINE, 2008, 14 (04) : 403 - 412
[4] A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of Lycium barbarum fruit (Goji)
Amagase, Harunobu
Farnsworth, Norman R.
[J]. FOOD RESEARCH INTERNATIONAL, 2011, 44 (07) : 1702 - 1717
[5] The Value of Vaccination
Bloom, David E.
[J]. HOT TOPICS IN INFECTION AND IMMUNITY IN CHILDREN VII, 2011, 697 : 1 - 8
[6] Optimization on conditions of Lycium barbarum polysaccharides liposome by RSM and its effects on the peritoneal macrophages function
Bo, Ruonan
Ma, Xia
Feng, Yibo
Zhu, Qian
Huang, Yee
Liu, Zhenguang
Liu, Cui
Gao, Zhenzhen
Hu, Yuanliang
Wang, Deyun
[J]. CARBOHYDRATE POLYMERS, 2015, 117 : 215 - 222
[7] Acetalated dextran is a chemically and biologically tunable material for particulate immunotherapy
Broaders, Kyle E.
Cohen, Joel A.
Beaudette, Tristan T.
Bachelder, Eric M.
Frechet, Jean M. J.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (14) : 5497 - 5502
[8] Ultrasmall Graphene Oxide Supported Gold Nanoparticles as Adjuvants Improve Humoral and Cellular Immunity in Mice
Cao, Yuhua
Ma, Yufei
Zhang, Mengxin
Wang, Haiming
Tu, Xiaolong
Shen, He
Dai, Jianwu
Guo, Huichen
Zhang, Zhijun
[J]. ADVANCED FUNCTIONAL MATERIALS, 2014, 24 (44) : 6963 - 6971
[9] DODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: A novel adjuvant and delivery system
Carneiro, Catarina
Correia, Alexandra
Collins, Tony
Vilanova, Manuel
Pais, Celia
Gomes, Andreia C.
Oliveira, M. Elisabete C. D. Real
Sampaio, Paula
[J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2015, 89 : 190 - 200
[10] Activation of Macrophages by Polysaccharide-protein Complex from Lycium barbarum L.
Chen, Zhisong
Soo, Mei Yun
Srinivasan, Nalini
Tan, Benny Kwong Huat
Chan, Soh Ha
[J]. PHYTOTHERAPY RESEARCH, 2009, 23 (08) : 1116 - 1122

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