Evaluation of low-pressure gas chromatography linked to ion-trap tandem mass spectrometry for the fast trace analysis of multiclass pesticide residues

被引:32
作者
González-Rodríguez, MJ [1 ]
Garrido-Frenich, A [1 ]
Arrebola, FJ [1 ]
Martínez-Vidal, JL [1 ]
机构
[1] Univ Almeria, Dept Analyt Chem, Almeria 04120, Spain
关键词
D O I
10.1002/rcm.707
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rapid multiresidue method for the analysis of 72 pesticides has been developed using a single injection with low-pressure gas chromatography/tandem mass spectrometry (LP-GC/MS/MS). The LP-GC/MS/MS method used a short capillary column of 10 m x 0.53 mm i.d. x 0.25 mum film thickness coupled with a 0.6 m x 0.10 mm i.d. restriction at the inlet end. Optimal LP-GC conditions were determined which achieved the fastest separation in MS/MS detection mode. Also MS/MS conditions were optimized in order to increase sensitivity and selectivity. The analytical parameters of the LP-GC/MS/MS method were compared with those obtained by GC/MS/MS using a conventional capillary column (30 m x 0.25 mm i.d. x 0.25 mum film thickness). Better precision and sensitivity values were obtained with the LP-GC/MS/MS approach. The limits of detection (LOD) of the compounds ranged from 0.1 to 14.1 mug L-1 for LP-GC/MS/MS, lower than those obtained for conventional GC/MS/MS that ranged from 0.1 to 17.5 mug L-1. The peak widths obtained with the short column in LP-GC are similar to those obtained using conventional capillary GC columns, and the peaks can be successfully identified by MS/MS detection with the conventional scan speed of ion-trap instruments. In addition, the analysis time was significantly reduced with LP-GC/MS/MS (32 min) versus GC/MS/MS (72 min), allowing the number of samples analyzed per day in a routine laboratory to be doubled. Copyright (C) 2002 John Wiley Sons, Ltd.
引用
收藏
页码:1216 / 1224
页数:9
相关论文
共 40 条
  • [1] Megabore versus microbore as the optimal column for fast gas chromatography mass spectrometry
    Amirav, A
    Tzanani, N
    Wainhaus, SB
    Dagan, S
    [J]. EUROPEAN MASS SPECTROMETRY, 1998, 4 (01): : 7 - 13
  • [2] Conventional inner diameter short capillary columns: an approach to speeding up gas chromatographic analysis of medium complexity samples
    Bicchi, C
    Brunelli, C
    Galli, M
    Sironi, A
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2001, 931 (1-2) : 129 - 140
  • [3] Blumberg LM, 1997, HRC-J HIGH RES CHROM, V20, P679
  • [4] Blumberg LM, 1997, HRC-J HIGH RES CHROM, V20, P597
  • [5] Blumberg LM, 1999, HRC-J HIGH RES CHROM, V22, P403
  • [6] BUSH KL, 1998, MASS SPECTROMETRY MA
  • [7] Strategies for speed optimisation in gas chromatography: an overview
    Cramers, CA
    Leclercq, PA
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 842 (1-2) : 3 - 13
  • [8] INCREASED SPEED OF ANALYSIS IN DIRECTLY COUPLED GAS CHROMATOGRAPHY-MASS SPECTROMETRY SYSTEMS - CAPILLARY COLUMNS AT SUB-ATMOSPHERIC OUTLET PRESSURES
    CRAMERS, CA
    SCHERPENZEEL, GJ
    LECLERCQ, PA
    [J]. JOURNAL OF CHROMATOGRAPHY, 1981, 203 (JAN): : 207 - 216
  • [9] High-speed gas chromatography: an overview of various concepts
    Cramers, CA
    Janssen, HG
    van Deursen, MM
    Leclercq, PA
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 856 (1-2) : 315 - 329
  • [10] Instrumentation and applications of fast high-resolution capillary gas chromatography
    David, F
    Gere, DR
    Scanlan, F
    Sandra, P
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 842 (1-2) : 309 - 319