The importance of virus-associated host ICAM-1 in human immunodeficiency virus type 1 dissemination depends on the cellular context

被引:34
作者
Bounou, S
Giguère, JF
Cantin, R
Gilbert, C
Imbeault, M
Martin, G
Tremblay, MJ
机构
[1] CHU Laval, Res Ctr, Ctr Infect Dis, Lab Human Immunoretrovirol, Quebec City, PQ G1V 4G2, Canada
[2] Univ Laval, Fac Med, Quebec City, PQ G1K 7P4, Canada
关键词
HIV; AIDS; monocytes; macrophages; T lymphocytes; dendritic cells; lymphoid tissue;
D O I
10.1096/fj.04-1755fje
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The primary objective of this study was to define whether the nature of virion-bound host cell membrane proteins influenced the process of human immunodeficiency virus 1 (HIV-1) capture and transmission. We pulsed cells of monocytoid lineage (established and primary) and CD4-negative epithelial cells transiently expressing DC-SIGN or LFA-1 with isogenic HIV-1 particles either devoid or bearing host-derived ICAM-1 or ICAM-3 before incubation with an indicator cell line. To our surprise, the ICAM-1/LFA-1 association was a more efficient transmission factor than the combined gp120/DC-SIGN and ICAM-3/DC-SIGN interactions. The involvement of the association between virus-bound ICAM-1 and its natural ligand LFA-1 in virus binding and carriage was confirmed when using more physiological cellular targets, i.e., human lymphoid tissues cultured ex vivo. However, the contribution of virus-anchored host ICAM-1 to the process of retention and transmission of HIV-1 could not be confirmed when using primary human cells of macrophage/dendritic lineage as transmitter cells and autologous CD4(+) T lymphocytes as targets. Altogether these data underscore the complexity of factors participating in virus-cell contact and efficient dissemination of HIV-1 to target cells.
引用
收藏
页码:1294 / +
页数:24
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