Interaction of Isoquinoline Alkaloids with Polymorphic DNA Structures

被引：15

作者：

Bhadra, Kakali ^{[1
]}

Maiti, Motilal ^{[1
]}

Kumar, Gopinatha Suresh ^{[1
]}

机构：

[1] Indian Inst Chem Biol, Council Sci & Ind Res, Biophys Chem Lab, Kolkata 700032, W Bengal, India

来源：

CHEMISTRY & BIODIVERSITY | 2009年 / 6卷 / 09期

关键词：

HANDED Z-DNA; DEOXYRIBONUCLEIC-ACID STRUCTURES; BETA-D-GLUCOSIDE; TOPOISOMERASE-I; LOW-PH; COOPERATIVE BINDING; BERBERINE CHLORIDE; INTERCALATION SITE; MOLECULAR ASPECTS; FORM DNA;

D O I：

10.1002/cbdv.200900017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of berberine, palmatine, and coralyne with the B, Z, and H-L form of poly[d(G-C)] was studied. Berberine and palmatine showed moderate binding to the B form, while coralyne showed higher binding, as revealed from spectroscopic and thermodynamic data. Berberine and coralyne binding to the B form was exothermic and enthalpy-driven, while palmatine showed exothermic binding which was favored by both negative enthalpy and negative entropy changes. Berberine and palmatine neither bind nor converted the Z-form structure to B form. Coralyne, on the other hand, exhibited a strong binding affinity to Z DNA structure that was enthalpy-driven. Berberine binding to the H-L form was cooperative, exothermic, and favored by both negative enthalpy and negative entropy changes with the formation of an induced CD band. Palmatine showed weak binding, while coralyne showed a strong binding with the H-L form. The structural differences in the isoquinoline alkaloids appear to influence the affinity and mode of interactions with these polymorphic DNA structures.

引用

页码：1323 / 1342

页数：20

共 63 条

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