Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography

被引：74

作者：

Bin Han, Quan ^{[1
]}

Song, Jing Zheng ^{[1
]}

Qiao, Chun Feng ^{[1
]}

Wong, Lina ^{[1
]}

Xu, Hong Xi ^{[1
]}

机构：

[1] Hong Kong Jockey Club Inst Chinese Med, Chinese Med Lab, Sha Tin, Hong Kong, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1127卷 / 1-2期

关键词：

recycling counter-current chromatography; preparative separation; Garcinia hanburyi; epimer; gambogic acid; epigambogic acid;

D O I：

10.1016/j.chroma.2006.07.044

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4: 1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their H-1 NMR and C-13 NMR spectra. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：298 / 301

页数：4

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