Characterization of ovarian carbonyl reductase gene expression during ovulation in the gonadotropin-primed immature rat

被引:32
作者
Espey, LL [1 ]
Yoshioka, S
Russell, D
Ujioka, T
Vladu, B
Skelsey, M
Fujii, S
Okamura, H
Richards, JS
机构
[1] Trinity Univ, Dept Biol, San Antonio, TX 78212 USA
[2] Baylor Coll Med, Dept Cell Biol, Houston, TX 77030 USA
[3] Kyoto Univ, Sch Med, Dept Gynecol & Obstet, Kyoto 606, Japan
[4] Kumamoto Univ, Sch Med, Dept Obstet & Gynecol, Kumamoto 860, Japan
关键词
D O I
10.1095/biolreprod62.2.390
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this differential-display polymerase chain reaction-based study, four different primer sets generated cDNA fragments of ovarian carbonyl reductase genes that were uniquely expressed during the ovulatory process in eCG-primed immature rats, The temporal pattern of expression of this aldo-keto reductase gene was delineated by extracting ovarian RNA at 0, 2, 4, 8, 12, and 24 h after induction of ovulation via injection of the primed animals with hCG, The results showed that at least four homologous forms of this gene were transcribed during ovulation. Northern blot analyses indicated a 14-fold increase in ovarian mRNA for carbonyl reductase, with expression reaching a peak at 8 h after hCG treatment and then declining to negligible levels during the next 16 h. In situ hybridization revealed that mast of the transcription was in the thecal connective tissue of the ovary and was absent from the granulosa layer of ovarian follicles. Treatment of the animals with ovulation-blocking doses of epostane (an inhibitor of progesterone synthesis) or indomethacin (an inhibitor of prostanoid synthesis) did not reduce the expression of ovarian carbonyl reductase. Nevertheless, the temporal pattern of expression of carbonyl reductase after the induction of ovulation suggests that this enzyme activity is at Least indirectly associated with the ovulatory process.
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页码:390 / 397
页数:8
相关论文
共 23 条
[1]   Identification of two closely related genes, inducible and noninducible carbonyl reductases in the rat ovary [J].
Aoki, H ;
Okada, T ;
Mizutani, T ;
Numata, Y ;
Minegishi, M ;
Miyamoto, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 230 (03) :518-523
[2]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[3]  
Espey L. L., 1997, Biology of Reproduction, V56, P178
[4]  
Espey Lawrence L., 1994, P725
[5]   OVARIAN HYDROXYEICOSATETRAENOIC ACIDS COMPARED WITH PROSTANOIDS AND STEROIDS DURING OVULATION IN RATS [J].
ESPEY, LL ;
TANAKA, N ;
ADAMS, RF ;
OKAMURA, H .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (02) :E163-E169
[6]   EFFECTS OF EPOSTANE ON OVARIAN LEVELS OF PROGESTERONE, 17-BETA-ESTRADIOL, PROSTAGLANDIN-E2, AND PROSTAGLANDIN-F2-ALPHA DURING OVULATION IN THE GONADOTROPIN-PRIMED IMMATURE RAT [J].
ESPEY, LL ;
ADAMS, RF ;
TANAKA, N ;
OKAMURA, H .
ENDOCRINOLOGY, 1990, 127 (01) :259-263
[7]   CLONING AND EXPRESSION OF THE CDNA-ENCODING RABBIT LIVER CARBONYL REDUCTASE [J].
GONZALEZ, B ;
SAPRA, A ;
RIVERA, H ;
KAPLAN, WD ;
YAM, B ;
FORREST, GL .
GENE, 1995, 154 (02) :297-298
[8]  
Inazu N, 1996, J REPROD FERTIL, V108, P123, DOI 10.1530/jrf.0.1080123
[9]  
Inazu N, 1998, J REPROD FERTIL, V112, P115, DOI 10.1530/jrf.0.1120115
[10]  
Inazu N, 1997, RES COMMUN MOL PATH, V98, P325