Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome

被引:121
作者
Blot, Nicolas
Mavathur, Ramesh
Geertz, Marcel
Travers, Andrew
Muskhelishvili, Georgi
机构
[1] Int Jacobs Univ Bremen, D-28759 Bremen, Germany
[2] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
基金
英国医学研究理事会;
关键词
FIS; H-NS; supercoiling; transcription regulation; metabolism;
D O I
10.1038/sj.embor.7400729
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Regulation of cellular growth implies spatiotemporally coordinated programmes of gene transcription. A central question, therefore, is how global transcription is coordinated in the genome. The growth of the unicellular organism Escherichia coli is associated with changes in both the global superhelicity modulated by cellular topoisomerase activity and the relative proportions of the abundant DNA-architectural chromatin proteins. Using a DNA-microarray-based approach that combines mutations in the genes of two important chromatin proteins with induced changes of DNA superhelicity, we demonstrate that genomic transcription is tightly associated with the spatial distribution of supercoiling sensitivity, which in turn depends on chromatin proteins. We further demonstrate that essential metabolic pathways involved in the maintenance of growth respond distinctly to changes of superhelicity. We infer that a homeostatic mechanism organizing the supercoiling sensitivity is coordinating the growth-phase-dependent transcription of the genome.
引用
收藏
页码:710 / 715
页数:6
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