Studies of aberrant phyllotaxy1 Mutants of Maize Indicate Complex Interactions between Auxin and Cytokinin Signaling in the Shoot Apical Meristem

被引:113
作者
Lee, Byeong-ha [1 ,2 ]
Johnston, Robyn [1 ]
Yang, Yan [1 ,3 ]
Gallavotti, Andrea [1 ,4 ]
Kojima, Mikiko [5 ]
Travencolo, Bruno A. N. [6 ]
Costa, Luciano da F. [6 ]
Sakakibara, Hitoshi [5 ]
Jackson, David [1 ]
机构
[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
[2] Sogang Univ, Dept Life Sci, Seoul 121742, South Korea
[3] Univ Amsterdam, NL-1098 SM Amsterdam, Netherlands
[4] Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA
[5] RIKEN, Plant Sci Ctr, Yokohama, Kanagawa 2300045, Japan
[6] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Paulo, Brazil
基金
美国国家科学基金会;
关键词
ARABIDOPSIS-THALIANA; RESPONSE REGULATORS; HOMEOBOX GENES; PIN PROTEINS; STEM-CELLS; TRANSPORT; INITIATION; PLANTS; PHYLLOTAXIS; METABOLISM;
D O I
10.1104/pp.109.137034
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
One of the most fascinating aspects of plant morphology is the regular geometric arrangement of leaves and flowers, called phyllotaxy. The shoot apical meristem (SAM) determines these patterns, which vary depending on species and developmental stage. Auxin acts as an instructive signal in leaf initiation, and its transport has been implicated in phyllotaxy regulation in Arabidopsis (Arabidopsis thaliana). Altered phyllotactic patterns are observed in a maize (Zea mays) mutant, aberrant phyllotaxy1 (abph1, also known as abphyl1), and ABPH1 encodes a cytokinin-inducible type A response regulator, suggesting that cytokinin signals are also involved in the mechanism by which phyllotactic patterns are established. Therefore, we investigated the interaction between auxin and cytokinin signaling in phyllotaxy. Treatment of maize shoots with a polar auxin transport inhibitor, 1-naphthylphthalamic acid, strongly reduced ABPH1 expression, suggesting that auxin or its polar transport is required for ABPH1 expression. Immunolocalization of the PINFORMED1 (PIN1) polar auxin transporter revealed that PIN1 expression marks leaf primordia in maize, similarly to Arabidopsis. Interestingly, maize PIN1 expression at the incipient leaf primordium was greatly reduced in abph1 mutants. Consistently, auxin levels were reduced in abph1, and the maize PIN1 homolog was induced not only by auxin but also by cytokinin treatments. Our results indicate distinct roles for ABPH1 as a negative regulator of SAM size and a positive regulator of PIN1 expression. These studies highlight a complex interaction between auxin and cytokinin signaling in the specification of phyllotactic patterns and suggest an alternative model for the generation of altered phyllotactic patterns in abph1 mutants. We propose that reduced auxin levels and PIN1 expression in abph1 mutant SAMs delay leaf initiation, contributing to the enlarged SAM and altered phyllotaxy of these mutants.
引用
收藏
页码:205 / 216
页数:12
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